Liu Yu, Liu Zhe, Gong Aijun, Qiu Lina, Zhang Weiwei, Li Jingrui, Li Fukai, Bai Yuzhen, Li Jiandi, Gao Ge
School of Chemistry and Biological Engineering, University of Science and Technology Beijing Beijing 100083 China
Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing Beijing 100083 China.
RSC Adv. 2019 Aug 12;9(43):25048-25055. doi: 10.1039/c9ra00664h. eCollection 2019 Aug 8.
Decabromodiphenyl ether (BDE-209) is a brominated flame retardant widely used in the world which, being an emerging persistent organic pollutant, poses a great potential hazard to both human health and the ecological environment. Microbial biodegradation has been considered as an effective and environment-friendly technique to remediate BDE-209. , a Gram-negative bacterium capable of degrading BDE-209, was isolated from PBDEs-contaminated soil. To promote microbial biodegradation of BDE-209 and gain further insight into its mechanism, cell changes and differential proteomic analysis of during biodegradation were studied. The results showed that high cell surface hydrophobicity of make the bacteria absorb BDE-209 more easily. The increase in cell membrane permeability was caused by the responding to BDE-209 stress. IR spectra showed that hydroxyl, amide and CH groups in the cell surface were involved in the interactions between BDE-209 with . The apoptotic-like cell changes and cell surface morphology changes were observed by flow cytometry (FCM) and field emission scanning electron microscopy (FESEM), respectively. Differentially expressed protein was analysed by two-dimensional electrophoresis (2-DE) and 40 protein spots were identified to be different after 5 days biodegradation.
十溴二苯醚（BDE - 209）是一种在全球广泛使用的溴化阻燃剂，作为一种新出现的持久性有机污染物，对人类健康和生态环境都构成了巨大的潜在危害。微生物生物降解被认为是修复BDE - 209的一种有效且环境友好的技术。从多溴二苯醚污染的土壤中分离出了一种能够降解BDE - 209的革兰氏阴性菌。为了促进BDE - 209的微生物生物降解并进一步深入了解其机制，研究了该菌在生物降解过程中的细胞变化和差异蛋白质组学分析。结果表明，该菌较高的细胞表面疏水性使其更容易吸收BDE - 209。细胞膜通透性的增加是该菌对BDE - 209胁迫作出反应所致。红外光谱表明，该菌细胞表面的羟基、酰胺基和CH基团参与了BDE - 209与该菌之间的相互作用。分别通过流式细胞术（FCM）和场发射扫描电子显微镜（FESEM）观察到了凋亡样细胞变化和细胞表面形态变化。通过二维电泳（2 - DE）分析差异表达蛋白，在生物降解5天后鉴定出40个蛋白点存在差异。
