Wiktor-Jedrzejczak W, Ratajczak M Z, Szczylik C
Department of Immunology, Central Clinical Hospital CSK WAM, Warsaw.
Arch Immunol Ther Exp (Warsz). 1989;37(3-4):261-7.
A modified polysaccharide-ditrizoate gradient centrifugation method is described. One hundred ml aliquots of marrow mixed 1:1 with medium is layered on 70 ml of polysaccharide-ditrizoate solution of specific density 1.077 in 5 x 19 cm flasks and centrifuged at 400 x g for 20 minutes at 20 degrees C. Interphase cells are collected and used for the transplant. The procedure is closed which reduces the danger of contamination, simple and takes between 2 and 3 hours. Two allogeneic HLA-identical transplants were performed using this technique and both resulted in full hematopoietic reconstitution and long term survival.
描述了一种改良的多糖-泛影酸盐梯度离心法。将100毫升与培养基按1:1混合的骨髓等分试样铺在5个19厘米长的烧瓶中70毫升比重为1.077的多糖-泛影酸盐溶液上，在20℃下以400×g离心20分钟。收集界面细胞用于移植。该操作是封闭的，减少了污染风险，简单且耗时2至3小时。使用该技术进行了两次 HLA 全相合的同种异体移植，均实现了完全的造血重建和长期存活。
