Mayer G, Wernet D, Northoff H, Schneider W
Department of Transfusion Medicine, University of Tübingen, FRG.
Vox Sang. 1994;66(2):112-6. doi: 10.1111/j.1423-0410.1994.tb00291.x.
A simple technique for red blood cell (RBC) removal in major ABO-incompatible bone marrow transplantation is reported requiring two centrifugation steps, special blood bags and a mechanical device to separate the buffy coat from RBCs within the bag. In 42 transplantations an average of 84% of nucleated cells was recovered with an average contamination of 7.5 ml packed RBCs. The preparations were well tolerated in all patients whose isoagglutinin titers had not been reduced. Bone marrow engraftment was not significantly different from control groups.
报道了一种用于主要ABO血型不相容骨髓移植中去除红细胞（RBC）的简单技术，该技术需要两个离心步骤、特殊血袋以及一种机械装置，以便在袋内将 Buffy 层与红细胞分离。在42例移植中，平均回收了84%的有核细胞，平均污染7.5毫升压积红细胞。所有未降低同种凝集素滴度的患者对这些制剂耐受性良好。骨髓植入情况与对照组无显著差异。
