Zhang Zhuo, Wang Bin, Hu Shouping, Zhang Jiaoer, He Xijun, Zheng Shimin
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030 Heilongjiang China ; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001 Heilongjiang China.
Virusdisease. 2015 Sep;26(3):203-6. doi: 10.1007/s13337-015-0265-9. Epub 2015 Jul 28.
In this study, a loop-mediated isothermal amplification (LAMP) assay was developed and optimized for the detection of Aleutian disease virus (ADV) in minks. The amplification could be completed within 45 min under isothermal condition by employing a set of six ADV genome-specific primers. The amplification results could be visualized directly with the naked eye by using fluorescent dye. Comparative experiments showed that the LAMP assay is superior to conventional polymerase chain reaction for the detection of both experimental and field samples. Results of current study indicated that the LAMP assay is a rapid and reliable technique for routine diagnosis of ADV infection in minks.
在本研究中,开发并优化了一种环介导等温扩增(LAMP)检测方法,用于检测水貂中的阿留申病病毒(ADV)。通过使用一组六条ADV基因组特异性引物,在等温条件下45分钟内即可完成扩增。使用荧光染料可直接用肉眼观察扩增结果。对比实验表明,在检测实验样本和现场样本时,LAMP检测方法优于传统聚合酶链反应。当前研究结果表明,LAMP检测方法是一种用于水貂ADV感染常规诊断的快速且可靠的技术。