Prieto Alberto, Díaz-Cao José Manuel, Fernández-Antonio Ricardo, Panadero Rosario, Díaz Pablo, López Ceferino, Morrondo Patrocinio, Díez-Baños Pablo, Fernández Gonzalo
Department of Animal Pathology (INVESAGA Group), Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain.
Department of Animal Pathology (INVESAGA Group), Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002 Lugo, Spain.
Vet Microbiol. 2014 Oct 10;173(3-4):355-9. doi: 10.1016/j.vetmic.2014.07.024. Epub 2014 Aug 15.
The Aleutian Mink Virus (AMDV) causes the Aleutian Mink Disease (AMD) or Mink Plasmacytosis, a disease responsible of high economic losses for industry worldwide. Despite there is evidence of the environmental persistence of the virus, there is not literature on the detection of this virus in environmental samples in farms and this fact would have great importance in the control programs of the disease. In order to detect contamination caused by AMDV on farms, several environmental samples were taken and examined using qPCR. 93.9% of samples taken from farms confirmed to be infected tested positive. The virus was also detected on a farm which, despite having no previous positive results, was sharing personnel with an infected farm. All samples taken from AMD-free farms tested negative, including a farm where an eradication procedure by stamping out had been performed during the preceding months. Higher contamination levels were observed in samples from those surfaces in direct contact with animals. These results are the first demonstration of environmental contamination in farms, hitherto suggested by epidemiological evidences, caused by AMDV on surfaces, furniture and equipments inside mink farms. qPCR is an useful tool for evaluating the spread of AMDV into the environment, and it may have important applications within the disease control programs.
阿留申水貂病毒（AMDV）会引发阿留申水貂病（AMD）或水貂浆细胞增多症，这种疾病给全球水貂养殖行业造成了巨大经济损失。尽管有证据表明该病毒能在环境中持续存在，但尚无关于在养殖场环境样本中检测该病毒的文献，而这一事实对于疾病防控计划具有重要意义。为了检测养殖场中AMDV造成 的污染，采集了多个环境样本并采用定量聚合酶链反应（qPCR）进行检测。从已确诊感染的养殖场采集的样本中，93.9%检测呈阳性。在一个此前检测结果均为阴性，但与一个受感染养殖场有人员往来的养殖场中，也检测到了该病毒。从无AMD养殖场采集的所有样本检测均为阴性，包括一个在前几个月已通过扑杀进行根除程序的养殖场。在与动物直接接触的表面采集的样本中，观察到了更高的污染水平。这些结果首次证明了养殖场环境受到污染，此前流行病学证据已表明，水貂养殖场内的表面、家具和设备受到了AMDV污染。qPCR是评估AMDV在环境中传播情况的有用工具，在疾病防控计划中可能具有重要应用。
