Haenen Steven, Vanoirbeek Jeroen A J, De Vooght Vanessa, Schoofs Liliane, Nemery Benoit, Clynen Elke, Hoet Peter H M
Centre for Environment and Health, KU Leuven, Leuven, Belgium; Research Group Functional Genomics and Proteomics, KU Leuven, Leuven, Belgium.
Centre for Environment and Health, KU Leuven, Leuven, Belgium.
PLoS One. 2015 Sep 23;10(9):e0138791. doi: 10.1371/journal.pone.0138791. eCollection 2015.
The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naïve mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the "sensitized signature" of B lymphocytes comparing TDI-sensitized mice with control mice.
Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses.
This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.
B淋巴细胞在化学物质诱发的哮喘中的作用尚不清楚。最近的研究表明，将来自甲苯二异氰酸酯（TDI）致敏小鼠的B淋巴细胞转移到未致敏小鼠、B细胞敲除小鼠和重症联合免疫缺陷（SCID）小鼠体内，在随后进行TDI激发后，这些小鼠会引发类似哮喘的反应。我们应用二维差异凝胶电泳（2D-DIGE）来描述与对照小鼠相比，TDI致敏小鼠B淋巴细胞的“致敏特征”。
鉴定出16种在致敏小鼠B淋巴细胞中显著上调或下调的蛋白质。特别是亲环蛋白A、丝切蛋白1和含CCHC结构域锌指蛋白11的表达差异，可能与B淋巴细胞作为非T淋巴细胞依赖性类似哮喘反应启动子的功能相关。
本研究揭示了化学物质诱发哮喘小鼠模型中致敏B细胞蛋白质组的重要变化，这将对B细胞功能产生重要影响。
Zotero 插件
