具有复制能力的乙型流感病毒报告基因病毒作为筛选抗病毒药物和抗体的工具
Replication-Competent Influenza B Reporter Viruses as Tools for Screening Antivirals and Antibodies.
作者信息
Fulton Benjamin O, Palese Peter, Heaton Nicholas S
机构信息
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
出版信息
J Virol. 2015 Dec;89(23):12226-31. doi: 10.1128/JVI.02164-15. Epub 2015 Sep 23.
Abstract
Influenza B virus is a human pathogen responsible for significant health and economic burden. Research into this pathogen has been limited by the lack of reporter viruses. Here we describe the development of both a replication-competent fluorescent influenza B reporter virus and bioluminescent influenza B reporter virus. Furthermore, we demonstrate these reporter viruses can be used to quickly monitor viral growth and permit the rapid screening of antiviral compounds and neutralizing antibodies.
摘要
乙型流感病毒是一种对健康和经济造成重大负担的人类病原体。对这种病原体的研究因缺乏报告病毒而受到限制。在此，我们描述了一种具有复制能力的荧光乙型流感报告病毒和生物发光乙型流感报告病毒的开发。此外，我们证明这些报告病毒可用于快速监测病毒生长，并允许对抗病毒化合物和中和抗体进行快速筛选。
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