Jain Dilpesh, Gangshettiwar Arti
Department of Pharmacology, Sinhgad College of Pharmacy, Vadgaon (Bk.), Pune, Maharashtra, India.
J Intercult Ethnopharmacol. 2014 Oct-Dec;3(4):186-91. doi: 10.5455/jice.20140903012921. Epub 2014 Sep 12.
The present study investigates synergistic effect of combination treatment of lycopene, quercetin and poloxamer188 in 3-nitropropionic acid (3-NP)-induced Huntington's disease (HD).
Anxiety and depression were induced in male Wistar rat by intra-peritoneal administration of 3-NP (10 mg/kg) for 14 days. Body weight was assessed on day 1, 7 and 14, whereas locomotion, anxiety and depression were assessed at the end of the experiment.
Administration of 3-NP induces HD like symptoms and produced a significant decrease in body weight on day 7 (P < 0.01)and day 14 (P < 0.001), further decreased locomotion, time spent and number of entries in light area as well as increased immobility period were observed. The rats treated with lycopene and quercetin alone significantly restore the body weight and locomotion count as well as alleviate anxiety and depression. However, combination treatment of lycopene and quercetin with and without poloxamer 188 produced more significant effect on body weight compared to Huntington control rats, but no significant effect was found between the treated groups. However, significant increased on locomotion, time spent and number of entries in light area and decreased immobility period was observed in the combination treated groups when compared to single drug therapy.
Combination treatment of lycopene and quercetin with and without poloxamer 188 in HD more effectively alleviate anxiety and depression than single drug therapy.
本研究探讨番茄红素、槲皮素和泊洛沙姆188联合治疗对3-硝基丙酸（3-NP）诱导的亨廷顿舞蹈病（HD）的协同作用。
通过腹腔注射3-NP（10毫克/千克），连续14天，诱导雄性Wistar大鼠产生焦虑和抑郁症状。在第1、7和14天评估体重，在实验结束时评估运动能力、焦虑和抑郁情况。
注射3-NP会诱发类似HD的症状，并导致第7天（P < 0.01）和第14天（P < 0.001）体重显著下降，还观察到运动能力进一步下降、在亮区停留的时间和进入亮区的次数减少，以及不动时间增加。单独用番茄红素和槲皮素治疗的大鼠能显著恢复体重和运动计数，并缓解焦虑和抑郁。然而，与亨廷顿病对照大鼠相比，无论是否添加泊洛沙姆188，番茄红素和槲皮素联合治疗对体重的影响更为显著，但各治疗组之间未发现显著差异。不过，与单一药物治疗相比，联合治疗组的运动能力显著增强、在亮区停留的时间和进入亮区的次数增加，不动时间减少。
在HD治疗中，无论是否添加泊洛沙姆188，番茄红素和槲皮素联合治疗比单一药物治疗更有效地缓解焦虑和抑郁。
