Kaur Navneet, Jamwal Sumit, Deshmukh Rahul, Gauttam Vinod, Kumar Puneet
Department of Pharmacology, ISF College of Pharmacy, Moga 142001, Punjab, India.
Research Scholar, Punjab Technical University, Jalandhar, India.
Toxicol Rep. 2015 Aug 12;2:1222-1232. doi: 10.1016/j.toxrep.2015.08.004. eCollection 2015.
Huntington's disease (HD) is a neurodegenerative disorder, characterized by progressive motor and non-motor dysfunction due to degeneration of medium spiny neurons in striatum. 3-Nitropropionic acid is commonly used to induce the animal model of HD. Rice bran is supposed to have beneficial effects on mitochondrial function. The present study has been designed to explore the effect of rice bran extract against 3-Nitropropionic acid induced neurotoxicity in rats. 3-Nitropropionic acid (10 mg/kg, i.p) was administered systemically for 21 days. Hexane and ethanol extract of rice bran were prepared using Soxhlation. Hexane (250 mg/kg) and ethanol extract (250 mg/kg) were administered per os for 21 days in 3-NP treated groups. Behavioral parameters (body weight, grip strength, motor coordination, locomotion) were conducted on 7th, 14th and 21st day. Animals were sacrificed on 22nd day for biochemical, mitochondrial dysfunction (Complex II), neuroinflammatory and neurochemical estimation in striatum. This study demonstrates significant alteration in behavioral parameters, oxidative burden (increased lipid peroxidation, nitrite concentration and decreased glutathione), mitochondrial function (decreased Complex II enzyme activity), pro-inflammatory mediators and neurochemical levels in 3-nitropropionic acid treated animals. Administration of hexane and ethanol extract prevented the behavioral, biochemical, neuroinflammatory (increased TNF-α, IL-1β and IL-6) and neurochemical alterations (decreased dopamine, norepinephrine, serotonin, 5-hydroxy indole acetic acid, GABA and increased 3,4-dihydro phenyl acetaldehyde, homovanillic acid and glutamate levels) induced by 3-nitropropionic acid. The outcomes of present study suggest that rice bran extract is beneficial and might emerge as an adjuvant or prophylactic therapy for treatment of HD like symptoms.
亨廷顿舞蹈症（HD）是一种神经退行性疾病，其特征是由于纹状体中中等棘状神经元的退化导致进行性运动和非运动功能障碍。3-硝基丙酸常用于诱导HD动物模型。米糠被认为对线粒体功能有有益作用。本研究旨在探讨米糠提取物对3-硝基丙酸诱导的大鼠神经毒性的影响。3-硝基丙酸（10mg/kg，腹腔注射）全身给药21天。采用索氏提取法制备米糠的己烷和乙醇提取物。在3-NP处理组中，己烷（250mg/kg）和乙醇提取物（250mg/kg）经口给药21天。在第7天、第14天和第21天进行行为参数（体重、握力、运动协调性、运动能力）测试。在第22天处死动物，以进行纹状体中的生化、线粒体功能障碍（复合物II）、神经炎症和神经化学评估。本研究表明，在3-硝基丙酸处理的动物中，行为参数、氧化负担（脂质过氧化增加、亚硝酸盐浓度升高和谷胱甘肽降低）、线粒体功能（复合物II酶活性降低）、促炎介质和神经化学水平有显著改变。给予己烷和乙醇提取物可预防3-硝基丙酸诱导的行为、生化、神经炎症（TNF-α、IL-1β和IL-6升高）和神经化学改变（多巴胺、去甲肾上腺素、5-羟色胺、5-羟吲哚乙酸、GABA降低，3,4-二氢苯乙醛、高香草酸和谷氨酸水平升高）。本研究结果表明，米糠提取物有益，可能成为治疗HD样症状的辅助或预防性疗法。
