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愈创蓝油烃对大鼠神经元和N2a神经母细胞瘤细胞的生化活性、细胞毒性及遗传毒性作用

Guaiazulene biochemical activity and cytotoxic and genotoxic effects on rat neuron and N2a neuroblastom cells.

作者信息

Togar Basak, Turkez Hasan, Hacimuftuoglu Ahmet, Tatar Abdulgani, Geyikoglu Fatime

机构信息

Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.

Department of Molecular Biology and Genetics, Faculty of Sciences, Erzurum Technical University, Erzurum, Turkey.

出版信息

J Intercult Ethnopharmacol. 2015 Jan-Mar;4(1):29-33. doi: 10.5455/jice.20141124062203. Epub 2015 Jan 3.

DOI:10.5455/jice.20141124062203
PMID:26401381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4566767/
Abstract

AIM

Neuroblastoma (NB)cells are often used in cancer researches such as glioblastoma cells since they have the potential of high mitotic activity, nuclear pleomorphism, and tumor necrosis. Guaiazulene (GYZ 1,4-dimethyl-7-isopropylazulene)is present in several essential oils of medicinal and aromatic plants. Many studies have reported the cytotoxic effect of GYZ; however, there are no studies that compare such effects between cancer cell lines and normal human cells after treatment with GYZ.

MATERIALS AND METHODS

In this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties (by 3-[4,5 dimetylthiazol -2-yl]-2,5 diphenlytetrazolium bromide [MTT] test), oxidative effects (by total antioxidant capacity [TAC] and total oxidative stress [TOS] analysis)and genotoxic damage potentials (by single cell gel electrophoresis)of GYZ.

RESULT

The results indicated that GYZ have anti-proliferative activity suppressing the proliferation of neuron and N2a-NB cells at high doses. In addition, GYZ treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells. On the other hand, the mean values of the total scores of cells showing DNA damage were not found different from the control values.

CONCLUSION

From this study, it is observed that GYZ has in vitro cytotoxic activity against neuron and N2a-NB cells.

摘要

目的

神经母细胞瘤（NB）细胞由于具有高有丝分裂活性、核多形性和肿瘤坏死的潜力，常用于癌症研究，如胶质母细胞瘤细胞。愈创木薁（GYZ，1,4 - 二甲基 - 7 - 异丙基薁）存在于多种药用和芳香植物的精油中。许多研究报道了GYZ的细胞毒性作用；然而，尚无研究比较用GYZ处理后癌细胞系和正常人细胞之间的这种作用。

材料与方法

在本研究中，我们旨在描述GYZ的体外抗增殖和/或细胞毒性特性（通过3 - [4,5 - 二甲基噻唑 - 2 - 基] - 2,5 - 二苯基四氮唑溴盐[MTT]试验）、氧化作用（通过总抗氧化能力[TAC]和总氧化应激[TOS]分析）以及遗传毒性损伤潜力（通过单细胞凝胶电泳）。

结果

结果表明，GYZ具有抗增殖活性，在高剂量时抑制神经元和N2a - NB细胞的增殖。此外，高剂量的GYZ处理导致神经元和N2a - NB细胞中TAC水平降低和TOS水平升高。另一方面，显示DNA损伤的细胞总评分平均值与对照值无差异。

结论

从本研究中观察到，GYZ对神经元和N2a - NB细胞具有体外细胞毒性活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/9e06cd830cda/JIE-4-29-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/be3b5b0fcd5d/JIE-4-29-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/8735eb1ff96d/JIE-4-29-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/31bc9dd7a616/JIE-4-29-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/9e06cd830cda/JIE-4-29-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/be3b5b0fcd5d/JIE-4-29-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/8735eb1ff96d/JIE-4-29-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/31bc9dd7a616/JIE-4-29-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/4566767/9e06cd830cda/JIE-4-29-g005.jpg

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