Chronscinski Denise, Cherukeri Srujana, Tan Fraser, Perfito Nicole, Lomax Joelle, Iorns Elizabeth
Noble Life Sciences, Inc. , Gaithersburg, MD , USA.
Science Exchange and The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative , Palo Alto, CA , USA.
PeerJ. 2015 Sep 15;3:e1231. doi: 10.7717/peerj.1231. eCollection 2015.
The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative (PCFMFRI) seeks to address growing concerns about reproducibility in scientific research by conducting replications of recent papers in the field of prostate cancer. This Registered Report describes the proposed replication plan of key experiments from "The Androgen Receptor Induces a Distinct Transcriptional Program in Castration-Resistant Prostate Cancer in Man" by Sharma and colleagues (2013), published in Cancer Cell in 2013. Of thousands of targets for the androgen receptor (AR), the authors elucidated a subset of 16 core genes that were consistently downregulated with castration and re-emerged with castration resistance. These 16 AR binding sites were distinct from those observed in cells in culture. The authors suggested that cellular context can have dramatic effects on downstream transcriptional regulation of AR binding sites. The present study will attempt to replicate Fig. 7C by comparing gene expression of the 16 core genes identified by Sharma and colleagues in xenograft tumor tissue compared to androgen treated LNCaP cells in vitro. The Prostate Cancer Foundation-Movember Foundation Reproducibility Initiative is a collaboration between the Prostate Cancer Foundation, the Movember Initiative, and Science Exchange, and the results of the replications will be published by PeerJ.
前列腺癌基金会-胡子月基金会可重复性倡议（PCFMFRI）旨在通过对前列腺癌领域近期论文进行重复实验，来应对科学界对研究可重复性日益增长的担忧。本注册报告描述了对夏尔马及其同事于2013年发表在《癌细胞》杂志上的论文《雄激素受体在去势抵抗性前列腺癌患者中诱导独特的转录程序》中的关键实验所提出的重复计划。在雄激素受体（AR）的数千个靶点中，作者阐明了一组16个核心基因，这些基因在去势后持续下调，并在去势抵抗时重新出现。这16个AR结合位点与在培养细胞中观察到的不同。作者认为细胞环境可能对AR结合位点的下游转录调控产生显著影响。本研究将通过比较夏尔马及其同事在异种移植肿瘤组织中鉴定出的16个核心基因的基因表达与体外雄激素处理的LNCaP细胞中的基因表达，来尝试重复图7C。前列腺癌基金会-胡子月基金会可重复性倡议是前列腺癌基金会、胡子月倡议和科学交流公司之间的合作，重复实验的结果将由PeerJ发表。
