阿尔茨海默病患者的脑脊液中含有真菌蛋白质和DNA。

Cerebrospinal Fluid from Alzheimer's Disease Patients Contains Fungal Proteins and DNA.

作者信息

Alonso Ruth, Pisa Diana, Rábano Alberto, Rodal Izaskun, Carrasco Luis

机构信息

Centro de Biología Molecular "Severo Ochoa", c/Nicolás Cabrera, 1, Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.

Department of Neuropathology and Tissue Bank, Unidad de Investigación Proyecto Alzheimer, Fundación CIEN, Instituto de Salud Carlos III, Madrid, Spain.

出版信息

J Alzheimers Dis. 2015;47(4):873-6. doi: 10.3233/JAD-150382.

DOI:10.3233/JAD-150382

PMID:26401766

Abstract

The identification of biomarkers for Alzheimer's disease is important for patient management and to assess the effectiveness of clinical intervention. Cerebrospinal fluid (CSF) biomarkers constitute a powerful tool for diagnosis and monitoring disease progression. We have analyzed the presence of fungal proteins and DNA in CSF from AD patients. Our findings reveal that fungal proteins can be detected in CSF with different anti-fungal antibodies using a slot-blot assay. Additionally, amplification of fungal DNA by PCR followed by sequencing distinguished several fungal species. The possibility that these fungal macromolecules could represent AD biomarkers is discussed.

摘要

阿尔茨海默病生物标志物的识别对于患者管理和评估临床干预效果至关重要。脑脊液（CSF）生物标志物是诊断和监测疾病进展的有力工具。我们分析了阿尔茨海默病患者脑脊液中真菌蛋白和DNA的存在情况。我们的研究结果表明，使用狭缝印迹法，不同的抗真菌抗体可在脑脊液中检测到真菌蛋白。此外，通过聚合酶链反应（PCR）扩增真菌DNA并进行测序可区分几种真菌物种。本文讨论了这些真菌大分子作为阿尔茨海默病生物标志物的可能性。