Yanagisawa I, Sakuma H, Shimura M, Wakamatsu Y, Yanagisawa S, Sairenji E
J Oral Implantol. 1989;15(3):168-77.
New objectives of the development of biomaterials in recent years include how to control surface characteristics of materials and the attachment of cells to implant sites. This study clarified the effect of "wettability" of materials on culture cells, with wettability being expressed by the contact angle of the material to the water. First, low-temperature plasma treatment was administered so that samples of the same materials and shapes could be obtained, differing only in wettability. The contact angles at the surfaces of the samples and their surface roughness were then measured, and surfaces were analyzed by ESCA. For clarification of the biological response of the cell to wettability, attachment of connective tissues and epithelial-tissue-originated established cell lines to the material and its cell spreading were investigated in each test sample. As a result, it was found that the contact angle of each material used in the experiment affected both the cell attachment and spreading rates; thus, wettability of biomaterials is considered to be an important parameter of biological effect at the cell level.
近年来生物材料发展的新目标包括如何控制材料的表面特性以及细胞在植入部位的附着。本研究阐明了材料的“润湿性”对培养细胞的影响，润湿性通过材料与水的接触角来表示。首先，进行低温等离子体处理，以便获得相同材料和形状的样品，仅润湿性不同。然后测量样品表面的接触角及其表面粗糙度，并通过光电子能谱分析表面。为了阐明细胞对润湿性的生物学反应，在每个测试样品中研究了结缔组织和上皮组织来源的已建立细胞系在材料上的附着及其细胞铺展情况。结果发现，实验中使用的每种材料的接触角都影响细胞附着和铺展速率；因此，生物材料的润湿性被认为是细胞水平上生物学效应的一个重要参数。
