Khang G, Jeon J H, Lee J W, Cho S C, Lee H B
Biomaterials Laboratories, Korea Research Institutes of Chemical Technology, Yusung, Taejon.
Biomed Mater Eng. 1997;7(6):357-68.
Surface treatment, such as plasma glow discharge treatment, onto poly(lactide-co-glycolide) (PLGA) has been investigated to improve the cell-, tissue- and blood-compatibility. Surface-treated samples were characterized by measurement with a contact angle goniometer and electron spectroscopy for chemical analysis (ESCA). The contact angles on the plasma-treated PLGA surfaces decreased with increasing plasma exposure time from 92 degrees to about 30 degrees, i.e., increased hydrophilicity. From the results of ESCA analysis of plasma-treated films, the incorporation of oxygen molecules into plasma onto the PLGA surface was observed. Cell adhesion and growth on plasma-treated PLGA surfaces were more active than on the control. Furthermore, the maximum adhesion and growth of cells in moderate hydrophilicity were investigated. Morphology of the adhered platelets on the plasma-treated PLGA surface showed less activity than on the control, and the number of adhered platelets on the plasma-exposed PLGA sample decreased with decreasing water contact angle. It seems that surface wettability of PLGA plays an important role in cell adhesion, spreading and growth, and platelet adhesion.
人们已经研究了诸如等离子体辉光放电处理等在聚（丙交酯-乙交酯）（PLGA）上的表面处理方法，以改善其细胞、组织和血液相容性。通过使用接触角测角仪测量以及化学分析电子能谱（ESCA）对表面处理后的样品进行了表征。等离子体处理的PLGA表面上的接触角随着等离子体暴露时间从92度增加到约30度而减小，即亲水性增加。从对等离子体处理薄膜的ESCA分析结果来看，观察到氧分子掺入到PLGA表面的等离子体中。在等离子体处理的PLGA表面上细胞的粘附和生长比在对照表面上更活跃。此外，还研究了中等亲水性条件下细胞的最大粘附和生长情况。等离子体处理的PLGA表面上粘附血小板的形态显示其活性低于对照表面，并且随着水接触角的减小，等离子体暴露的PLGA样品上粘附的血小板数量减少。看来PLGA的表面润湿性在细胞粘附、铺展和生长以及血小板粘附中起着重要作用。
