Rèpice F, Vannelli G B, Balboni G C
Arch Ital Anat Embriol. 1989 Oct-Dec;94(4):393-403.
The structure, the ultrastructure and the number of myonuclei and satellite cells in Duchenne's muscular dystrophy and in control muscles were compared in order to determine the possible changes in the satellite cells population. The bioptical fragments were obtained from 16 healthy (control) and from 16 dystrophic male children from 12 to 96 months of age. The biopsies were embedded in paraffin and in Durcupan and the sections were stained with ematossilin-eosin, P.A.S. for the light microscope observation and with uranil-acetate and lead-citrate for the electron microscope study. Moreover the semithin sections were stained according to the method of Ontell (1974) that is specific for the satellite cells identification. The morphological aspects of the dystrophic muscles are the same previously reported by other authors. The quantitative analysis of the myonuclei and satellite cells in control and dystrophic muscles was carried out on five random sections of each biopsy. The whole number of nuclei (myonuclei and satellite cell nuclei) and the number of the satellite cells nuclei were evaluated and the mean values in controls and dystrophic muscles were compared with the t Student test. The obtained results show that: 1) in the control muscles the satellite cells number is nearly the same in all ages considered; 2) in the dystrophic muscles the satellite cells number is in a statistically significant way greater than in control muscles and show a moderate trend to increase with aging; 3) in the dystrophic muscles the whole number of nuclei (myonuclei and satellite cells) is greater than in control in a statistically significant way and this increase is due to the number of satellite cells.
为了确定卫星细胞群体可能发生的变化，对杜兴氏肌营养不良症患者的肌肉和对照肌肉的结构、超微结构以及肌细胞核和卫星细胞的数量进行了比较。从16名年龄在12至96个月的健康（对照）男童和16名患肌营养不良症的男童身上获取活检组织切片。将活检组织包埋在石蜡和Durcupan中，切片分别用苏木精 - 伊红、PAS染色用于光学显微镜观察，用醋酸铀和柠檬酸铅染色用于电子显微镜研究。此外，半薄切片按照Ontell（1974年）的方法染色，该方法对卫星细胞识别具有特异性。营养不良肌肉的形态学特征与其他作者先前报道的相同。对每个活检组织的五个随机切片进行对照肌肉和营养不良肌肉中肌细胞核和卫星细胞的定量分析。评估细胞核总数（肌细胞核和卫星细胞核）和卫星细胞核数量，并通过t检验比较对照肌肉和营养不良肌肉中的平均值。获得的结果表明：1）在对照肌肉中，在所考虑的所有年龄段中卫星细胞数量几乎相同；2）在营养不良肌肉中，卫星细胞数量在统计学上显著高于对照肌肉，并且显示出随年龄增长而适度增加的趋势；3）在营养不良肌肉中，细胞核总数（肌细胞核和卫星细胞）在统计学上显著高于对照，并且这种增加是由于卫星细胞的数量。
