Zhang M, McLennan I S
Department of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand.
Muscle Nerve. 1994 Sep;17(9):987-94. doi: 10.1002/mus.880170905.
Satellite cells are essential for the growth and regeneration of skeletal muscles but the study of them has been difficult due to the need to use an electron microscope. This article describes a method which enables satellite cells to be identified with a light microscope. Satellite cells were distinguished from: (1) myonuclei on the basis of whether the nuclei were inside or outside the plasmalemma of a fiber (stained with antidystrophin); and (2) interstitial cells depending on whether they were inside or outside of a fiber's basal lamina (stained with anticollagen IV). Some satellite cells were stained by the antidystrophin which further aided their identification. The question of whether all satellite cells are myogenic has been investigated and discussed. This method will facilitate the study of satellite cells in biopsies of human muscles and the estimation of satellite cell number in laboratory animals.
卫星细胞对于骨骼肌的生长和再生至关重要，但由于需要使用电子显微镜，对它们的研究一直很困难。本文描述了一种能够用光学显微镜识别卫星细胞的方法。卫星细胞可与以下细胞区分开来：（1）肌细胞核，依据细胞核是在纤维的质膜内还是外（用抗肌萎缩蛋白染色）；（2）间质细胞，取决于它们是在纤维基底膜的内还是外（用抗IV型胶原染色）。一些卫星细胞被抗肌萎缩蛋白染色，这进一步有助于对它们的识别。关于是否所有卫星细胞都具有成肌性的问题已得到研究和讨论。该方法将有助于对人类肌肉活检中的卫星细胞进行研究，并有助于估计实验动物中的卫星细胞数量。
