Abiko Y, Shimono M
Bull Tokyo Dent Coll. 1989 Nov;30(4):195-204.
The purpose of the present study is to elucidate influences of diet on periodontal regeneration following experimental periodontitis in rats. S-D rats were divided into two groups, which were fed either a conventional diet (group A) or a sucrose-rich diet (group B). Experimental periodontitis was produced by placement of elastic bands between maxillary molar teeth. After one week, the elastic bands were removed and the animals were sacrified by perfusion fixation with formalin immediately, 1, 3, or 5 days, or 1, 2, 3, 4, 6, or 8 weeks after the removal. Sections of the teeth and surrounding tissue were examined by light microscopy. Three days after the removal, in both groups, granulation tissues with many inflammatory cells were covered by epithelial cells; the attachment site to the root surface was located more apically than the cemento-enamel junction. Distinct periodontal pockets were formed by five days after the removal in group A. In group B, the infiltrations of inflammatory cells were more prominent than in group A. Six weeks after the removal in both groups, an epithelium which resembled long junctional epithelium rather than pocket epithelium became thicker. Eight weeks after the removal, the findings were similar to those of six weeks in group A. However, a severe inflammatory cell concentration was found in group B. These results suggest that periodontal wound healing is markedly influenced by diet, and that this experimental system is useful for studying dynamic changes in both pocket and long junctional epithelia.
本研究的目的是阐明饮食对大鼠实验性牙周炎后牙周组织再生的影响。将Sprague-Dawley（S-D）大鼠分为两组，分别给予常规饮食（A组）或高糖饮食（B组）。通过在上颌磨牙间放置弹力带诱导实验性牙周炎。一周后，去除弹力带，并在去除后即刻、1天、3天或5天，或1周、2周、3周、4周、6周或8周对动物进行灌注固定处死。对牙齿及周围组织切片进行光学显微镜检查。去除弹力带3天后，两组均可见有许多炎症细胞的肉芽组织被上皮细胞覆盖；根面附着位点位于牙骨质-釉质界的根尖侧。去除弹力带5天后，A组形成明显的牙周袋。B组炎症细胞浸润比A组更显著。去除弹力带6周后，两组均可见类似长结合上皮而非袋上皮的上皮增厚。去除弹力带8周后，A组的结果与6周时相似。然而，B组发现有严重的炎症细胞聚集。这些结果表明，牙周创伤愈合明显受饮食影响，且该实验系统有助于研究牙周袋和长结合上皮的动态变化。
