Department of Food Science and Technology, Seoul Women's University, Seoul 139-774, Republic of Korea.
Kwang Dong Pharmaceutical Co., LTD., Gyeonggi-do 459-040, Republic of Korea.
J Photochem Photobiol B. 2015 Dec;153:164-72. doi: 10.1016/j.jphotobiol.2015.09.017. Epub 2015 Sep 24.
The aim of this study is to evaluate the effect of spent coffee ground (SCG) ethanol extract on UVB-induced skin aging in hairless mice. An ethanol extract of SCG (ESCG) was prepared using the residue remaining after extraction of oil from roasted SCG. High performance liquid chromatography (HPLC) analysis showed that the content of caffeine (41.58 ± 0.54 μg/mg) was higher than that of chlorogenic acid isomers (~9.17 μg/mg) in ESCG. ESCG significantly decreased the UVB-induced intracellular reactive oxygen species in HaCaT cells. UVB-induced wrinkle formation in mice dorsal skin was effectively reduced by ESCG administration; high dose of ESCG (5 g/L) caused the reduction of wrinkle area by 30% compared with UVB-treated control (UVBC). This result correlated with the ESCG-mediated decrease in epidermis thickness (25%). In addition, ESCG administration significantly reduced transdermal water loss (20%) and erythema formation (35%) derived from UVB exposure. Collagen type I (COL-1) level in dorsal skin was effectively recovered by ESCG administration. These results were supported by down-regulation of collagen-degrading matrix metalloproteinase 2 (MMP2) and 9 (MMP9) expressions. Our results indicate that ESCG protects mouse skin from UVB-induced photoaging by suppressing the expression of matrix metalloproteinases. Our study suggests that ESCG may be anti-photoaging agent.
本研究旨在评估咖啡渣（SCG）乙醇提取物对 UVB 诱导的无毛小鼠皮肤衰老的影响。使用从烤 SCG 中提取油后剩余的残渣制备 SCG 的乙醇提取物（ESCG）。高效液相色谱（HPLC）分析表明，ESCG 中咖啡因（41.58 ± 0.54 μg/mg）的含量高于绿原酸异构体（~9.17 μg/mg）。ESCG 可显著降低 HaCaT 细胞中 UVB 诱导的细胞内活性氧。ESCG 给药可有效减少小鼠背部皮肤的 UVB 诱导皱纹形成；与 UVB 处理对照组（UVBC）相比，高剂量 ESCG（5 g/L）可使皱纹面积减少 30%。这一结果与 ESCG 介导的表皮厚度减少（25%）相关。此外，ESCG 给药可显著减少由 UVB 暴露引起的经皮水分流失（20%）和红斑形成（35%）。ESCG 给药可有效恢复背部皮肤中 I 型胶原蛋白（COL-1）水平。这些结果得到了下调胶原降解基质金属蛋白酶 2（MMP2）和 9（MMP9）表达的支持。我们的研究结果表明，ESCG 通过抑制基质金属蛋白酶的表达来保护小鼠皮肤免受 UVB 诱导的光老化。我们的研究表明，ESCG 可能是一种抗光老化剂。
