Leivo Janne, Mäkelä Joonas, Rosenberg Jaana, Lamminmäki Urpo
University of Turku, Department of Biochemistry, Tykistökatu 6 A, FIN-20520, Turku, Finland.
University of Turku, Department of Biochemistry, Tykistökatu 6 A, FIN-20520, Turku, Finland.
Anal Biochem. 2016 Jan 1;492:27-9. doi: 10.1016/j.ab.2015.09.014. Epub 2015 Sep 26.
The occurrence of boar taint and the European Commission recommendation to discontinue the surgical castration of pigs by the year 2018 creates an urgent need for new analytical methods that are simple, affordable, and suitable for field testing. We describe the generation and engineering of a skatole-specific antibody derived from a synthetic antibody library and the development of ELISA for its detection. The immunoassay is capable of detecting skatole with IC50 of 222 μg L(-1), which is within the analytical threshold level suggested for skatole, and with low cross-reactivity interference from other indolic compounds.
公猪异味的出现以及欧盟委员会建议到2018年停止对猪进行手术阉割，这迫切需要新的分析方法，这些方法要简单、经济实惠且适用于现场检测。我们描述了一种从合成抗体库中获得的粪臭素特异性抗体的产生和工程改造，以及用于其检测的酶联免疫吸附测定（ELISA）的开发。该免疫测定法能够检测粪臭素，其半数抑制浓度（IC50）为222μg L⁻¹，处于粪臭素建议的分析阈值水平范围内，并且受到其他吲哚类化合物的交叉反应干扰较低。
