Loh Jacelyn M S, Proft Thomas
Department of Molecular Medicine & Pathology, School of Medical Sciences and the Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand.
Biotechnol Lett. 2016 Jan;38(1):139-43. doi: 10.1007/s10529-015-1967-1. Epub 2015 Sep 26.
To determine if multiple streptococcal species can be easily labeled for biophotonic imaging using a toxin-antitoxin stabilized reporter plasmid containing the native firefly luciferase gene, originally developed for use in Group A Streptococcus.
A number of streptococcal species including Group B Streptococcus, Group C Streptococcus, Group G Streptococcus, S. iniae, S. vestibularis, and S. salivarius were successfully transformed with the reporter plasmid. In absence of antibiotic selection, the plasmid had variable stability amongst the six strains. The expression of firefly luciferase was highest in Group B Streptococcus and S. iniae, as observed by the brightest signal and lowest detection limits in vitro.
Multiple streptococcal species can be easily transformed with our toxin-antitoxin stabilized bioluminescent reporter plasmid. However, this plasmid shows variable stability and signal in different species, restricting its use for certain applications.
使用最初为A群链球菌开发的、含有天然萤火虫荧光素酶基因的毒素 - 抗毒素稳定报告质粒，确定多种链球菌是否能被轻松标记用于生物光子成像。
包括B群链球菌、C群链球菌、G群链球菌、海豚链球菌、前庭链球菌和唾液链球菌在内的多种链球菌成功用报告质粒进行了转化。在没有抗生素选择的情况下，该质粒在六种菌株中的稳定性各不相同。如在体外观察到的最亮信号和最低检测限所示，萤火虫荧光素酶在B群链球菌和海豚链球菌中的表达最高。
我们的毒素 - 抗毒素稳定生物发光报告质粒能够轻松转化多种链球菌。然而，该质粒在不同物种中显示出不同的稳定性和信号，限制了其在某些应用中的使用。
