Wang Weiwei, Kratz Karl, Behl Marc, Yan Wan, Liu Yue, Xu Xun, Baudis Stefan, Li Zhengdong, Kurtz Andreas, Lendlein Andreas, Ma Nan
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Helmholtz Virtual Institute - Multifunctional Materials in Medicine, Berlin and Teltow, Teltow, Germany.
Clin Hemorheol Microcirc. 2015;61(2):301-21. doi: 10.3233/CH-152001.
Polyether ether ketone (PEEK) as a high-performance, thermoplastic implant material entered the field of medical applications due to its structural function and commercial availability. In bone tissue engineering, the combination of mesenchymal stem cells (MSCs) with PEEK implants may accelerate the bone formation and promote the osseointegration between the implant and the adjacent bone tissue. In this concept the question how PEEK influences the behaviour and functions of MSCs is of great interest. Here the cellular response of human adipose-derived MSCs to PEEK was evaluated and compared to tissue culture plate (TCP) as the reference material. Viability and morphology of cells were not altered when cultured on the PEEK film. The cells on PEEK presented a high proliferation activity in spite of a relatively lower initial cell adhesion rate. There was no significant difference on cell apoptosis and senescence between the cells on PEEK and TCP. The inflammatory cytokines and VEGF secreted by the cells on these two surfaces were at similar levels. The cells on PEEK showed up-regulated BMP2 and down-regulated BMP4 and BMP6 gene expression, whereas no conspicuous differences were observed in the committed osteoblast markers (BGLAP, COL1A1 and Runx2). With osteoinduction the cells on PEEK and TCP exhibited a similar osteogenic differentiation potential. Our results demonstrate the biofunctionality of PEEK for human MSC cultivation and differentiation. Its clinical benefits in bone tissue engineering may be achieved by combining MSCs with PEEK implants. These data may also provide useful information for further modification of PEEK with chemical or physical methods to regulate the cellular processes of MSCs and to consequently improve the efficacy of MSC-PEEK based therapies.
聚醚醚酮（PEEK）作为一种高性能热塑性植入材料，因其结构功能和商业可得性而进入医学应用领域。在骨组织工程中，间充质干细胞（MSCs）与PEEK植入物的结合可能会加速骨形成，并促进植入物与相邻骨组织之间的骨整合。在这一概念中，PEEK如何影响MSCs的行为和功能是一个备受关注的问题。在此，评估了人脂肪来源的MSCs对PEEK的细胞反应，并将其与作为参考材料的组织培养板（TCP）进行比较。在PEEK膜上培养时，细胞的活力和形态没有改变。尽管初始细胞粘附率相对较低，但PEEK上的细胞呈现出较高的增殖活性。PEEK上的细胞与TCP上的细胞在细胞凋亡和衰老方面没有显著差异。这两个表面上的细胞分泌的炎性细胞因子和VEGF水平相似。PEEK上的细胞显示骨形态发生蛋白2（BMP2）基因表达上调，骨形态发生蛋白4（BMP4）和骨形态发生蛋白6（BMP6）基因表达下调，而在成骨细胞特异性标志物（骨钙素（BGLAP）、I型胶原蛋白α1（COL1A1）和Runx2）方面未观察到明显差异。在成骨诱导下，PEEK和TCP上的细胞表现出相似的成骨分化潜能。我们的结果证明了PEEK在人MSCs培养和分化方面的生物功能。通过将MSCs与PEEK植入物结合，可能实现其在骨组织工程中的临床益处。这些数据也可能为通过化学或物理方法进一步修饰PEEK以调节MSCs的细胞过程，从而提高基于MSCs-PEEK疗法的疗效提供有用信息。
