Xiong Yi, Wang Jian, Wei Di, Zhao Jun, Ye Mei
Department of Emergency Medicine, Second Peopleγs Hospital of Zunyi City, Zunyi 563000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2015 Sep;17(9):942-5.
To observe the changes in Th17 cell levels in bronchoalveolar lavage fluid in children of sepsis with acute lung injury and the relationship between the Th17 cell levels and prognosis.
Fifty children of sepsis with acute lung injury were enrolled in the study. The percentages of Th17 cells in bronchoalveolar lavage fluid were measured by flow cytometry. The patients of sepsis with acute lung injury were classified into three groups based on the Pediatric Critical Illness Score (PCIS): extremely critical, critical and non-critical. According to the clinical prognosis, the patients were classified into survical and death groups. Th17 cell levels were compared between the two groups. The relationship between Th17 cell levels and the PCIS scores was analyzed.
With the increase in the severity of sepsis, Th17 cell levels in bronchoalveolar lavage fluid were gradually increased (P<0.05). The Th17 cell levels were negatively correlated to the PCIS scores (r=-0.853; P<0.01). The Th17 cell levels were significantly higher in the death group than in the survival group. Moreover, compared with the survival group, the PCIS scores were lower in the death group (P<0.05).
The increased Th17 cell levels in children of sepsis with acute lung injury are closely related to the severity and prognosis of patients, suggesting that Th17 cell levels can be used as a predictor of the severity and prognosis.
观察脓毒症合并急性肺损伤患儿支气管肺泡灌洗液中Th17细胞水平的变化及其与预后的关系。
选取50例脓毒症合并急性肺损伤患儿作为研究对象。采用流式细胞术检测支气管肺泡灌洗液中Th17细胞的百分比。根据小儿危重病例评分（PCIS）将脓毒症合并急性肺损伤患者分为极危重症组、重症组和非重症组。根据临床预后将患者分为存活组和死亡组。比较两组Th17细胞水平。分析Th17细胞水平与PCIS评分的关系。
随着脓毒症严重程度的增加，支气管肺泡灌洗液中Th17细胞水平逐渐升高（P<0.05）。Th17细胞水平与PCIS评分呈负相关（r=-0.853；P<0.01）。死亡组Th17细胞水平显著高于存活组。此外，与存活组相比，死亡组PCIS评分更低（P<0.05）。
脓毒症合并急性肺损伤患儿Th17细胞水平升高与患者的严重程度及预后密切相关，提示Th17细胞水平可作为严重程度及预后的预测指标。
