基于ITS序列的DNA条形码技术鉴别玛咖（玛卡独行菜）及其掺假品。

Identification of maca (Lepidium meyenii Walp.) and its adulterants by a DNA-barcoding approach based on the ITS sequence.

作者信息

Chen Jin-Jin, Zhao Qing-Sheng, Liu Yi-Lan, Zha Sheng-Hua, Zhao Bing

机构信息

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

出版信息

Chin J Nat Med. 2015 Sep;13(9):653-9. doi: 10.1016/S1875-5364(15)30062-5.

DOI:10.1016/S1875-5364(15)30062-5

PMID:26412424

Abstract

Maca (Lepidium meyenii) is an herbaceous plant that grows in high plateaus and has been used as both food and folk medicine for centuries because of its benefits to human health. In the present study, ITS (internal transcribed spacer) sequences of forty-three maca samples, collected from different regions or vendors, were amplified and analyzed. The ITS sequences of nineteen potential adulterants of maca were also collected and analyzed. The results indicated that the ITS sequence of maca was consistent in all samples and unique when compared with its adulterants. Therefore, this DNA-barcoding approach based on the ITS sequence can be used for the molecular identification of maca and its adulterants.

摘要

玛咖（Lepidium meyenii）是一种生长在高原地区的草本植物，由于其对人体健康有益，几个世纪以来一直被用作食物和民间药物。在本研究中，对从不同地区或供应商收集的43个玛咖样本的ITS（内转录间隔区）序列进行了扩增和分析。还收集并分析了19种玛咖潜在掺假品的ITS序列。结果表明，玛咖的ITS序列在所有样本中是一致的，并且与掺假品相比是独特的。因此，这种基于ITS序列的DNA条形码方法可用于玛咖及其掺假品的分子鉴定。