Dournon C, Demassieux C, Durand D, Lesimple M
Laboratoire de Biologie Expérimentale, Université de Nancy I, Vandoeuvre les Nancy, France.
Int J Dev Biol. 1989 Dec;33(4):477-85.
PGC counts were carried out on larvae of Pleurodeles waltl (urodele amphibia) issued from standard, monosexual male and monosexual female offspring while the genital ridges were settling. During this period, which is characterized by a zero mitotic index (and is therefore called the Po period), and which lasts from stage 35 to stage 41, no PGC proliferation occurs. A statistical analysis indicated that PGC counts per larva are sex genotype independent and that offspring may be divided into three groups with average PGC counts of 96.9, 51.0 and 31.1, respectively. A fourth group with an average of 18.3 PGCs has been identified using experimental larvae reared at 30 degrees C from stage 30. The PGC count of 96.9 would result from at least three mitotic cycles. Before the Po period, germ cells are not identifiable. A hypothesis concerning genetic control of PGC proliferation before Po was deduced from this analysis.
在标准、单性雄体和单性雌体后代的滑蹠螈（有尾两栖动物）幼体生殖嵴形成时，对其进行了原生殖细胞（PGC）计数。在这个时期，其特征是有丝分裂指数为零（因此称为Po期），从第35阶段持续到第41阶段，没有PGC增殖发生。统计分析表明，每个幼体的PGC计数与性别基因型无关，后代可分为三组，平均PGC计数分别为96.9、51.0和31.1。使用从第30阶段开始在30摄氏度饲养的实验幼体，确定了第四组平均有18.3个PGC。96.9的PGC计数至少来自三个有丝分裂周期。在Po期之前，生殖细胞无法识别。从该分析中推导了一个关于Po期之前PGC增殖的遗传控制的假设。
