Kwon Tae-Rin, Oh Chang Taek, Choi Eun Ja, Kim Soon Re, Jang Yu-Jin, Ko Eun Jung, Suh Daewoong, Yoo Kwang Ho, Kim Beom Joon
Department of Medicine, Graduate, School, Chung-Ang, University, Seoul, Korea.
Department of Dermatology, Chung-Ang, University, College of Medicine, Seoul, Korea.
Lasers Surg Med. 2015 Dec;47(10):824-32. doi: 10.1002/lsm.22425. Epub 2015 Sep 28.
Ultraviolet light-emitting diodes (UV-LEDs) are a novel light source for phototherapy. This research investigated the in vitro safety and efficacy of UV-LEDs as a phototherapeutic device for atopic dermatitis (AD).
STUDY DESIGN/MATERIALS AND METHODS: Human keratinocytes and fibroblasts were irradiated by UV-LEDs with a center wavelength of 310 and 340 nm. We examined the effects of UV-LED irradiation on the suppression of TNF-α/IFN-γ-induced activation of STAT1 and ICAM-1 and on NF-κB expression; we used the following methods: cell viability assay, reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, Western blotting, and immunocytochemistry.
We observed anti-inflammatory responses through the suppression of TNF-α/IFN-γ-induced expression of TARC and MCP-1/CCL2, IL-1beta, IL-6, and sICAM-1 via blockage of ICAM-1 activation and subsequent activation of STAT1 and NF-κB. The results suggested that UV-LED irradiation inhibited ICAM expression by suppressing TNF-α/IFN-γ-induced NF-κB activation in vitro.
We concluded that novel UV-LED (310 and 340 nm) modalities were effective for the treatment of AD and may be promising for the treatment of inflammatory skin diseases.
紫外线发光二极管（UV-LED）是一种用于光疗的新型光源。本研究调查了UV-LED作为特应性皮炎（AD）光疗设备的体外安全性和有效性。
研究设计/材料与方法：用中心波长为310和340nm的UV-LED照射人角质形成细胞和成纤维细胞。我们通过以下方法检测了UV-LED照射对抑制TNF-α/IFN-γ诱导的STAT1和ICAM-1激活以及对NF-κB表达的影响：细胞活力测定、逆转录-聚合酶链反应、酶联免疫吸附测定、蛋白质印迹法和免疫细胞化学。
我们观察到通过阻断ICAM-1激活以及随后激活STAT1和NF-κB，抑制TNF-α/IFN-γ诱导的TARC和MCP-1/CCL2、IL-1β、IL-6和sICAM-1表达，从而产生抗炎反应。结果表明，UV-LED照射在体外通过抑制TNF-α/IFN-γ诱导的NF-κB激活来抑制ICAM表达。
我们得出结论，新型UV-LED（310和340nm）模式对AD治疗有效，可能有望用于治疗炎症性皮肤病。
