用 Phanerochaete chrysosporium 细胞和来自 Trametes versicolor 的复合交联酶聚集体共固定化的 Ca-海藻酸盐颗粒。
A Ca-alginate particle co-immobilized with Phanerochaete chrysosporium cells and the combined cross-linked enzyme aggregates from Trametes versicolor.
机构信息
Qilu University of Technology, No. 3501, Daxue Rd, Changqing District, Jinan, Shandong Province 250353, China.
Qilu University of Technology, No. 3501, Daxue Rd, Changqing District, Jinan, Shandong Province 250353, China.
出版信息
Bioresour Technol. 2015 Dec;198:464-9. doi: 10.1016/j.biortech.2015.09.032. Epub 2015 Sep 21.
For improving stability of immobilized white-rot fungus to treat various effluents, Phanerochaete chrysosporium cells and the combined cross-link enzyme aggregates (combi-CLEAs) prepared from Trametes versicolor were co-immobilized into the Ca-alginate gel particles in this paper. The activity yields of obtained combi-CLEAs were 42.7% for lignin peroxidases (LiPs), 31.4% for manganese peroxidases (MnPs) and 40.4% for laccase (Lac), respectively. And their specific activities were 30.2U/g as combi-CLEAs-LiPs, 9.5 U/g as combi-CLEAs-MnPs and 28.4 U/g as combi-CLEAs-Lac. Further, the present of the combi-CLEAs in the particles extremely improved their ability to degrade the dyes. Compared to the immobilized Ph. chrysosporium without the combi-CLEAs, the co-immobilized particles enhanced the decolorized rate of Acid Violet 7 (from 45.2% to 93.4%) and Basic Fuchsin (from 12.1% to 67.9%). In addition, the addition of the combi-CLEAs improved the adaptability of the white-rot fungal particles to adverse environmental conditions.
为了提高固定化白腐真菌处理各种废水的稳定性，本文将糙皮侧耳（Trametes versicolor）细胞与交联酶聚集体（combi-CLEAs）共同固定在海藻酸钙凝胶颗粒中。所得到的 combi-CLEAs 的酶活收率分别为木质素过氧化物酶（LiPs）42.7%、锰过氧化物酶（MnPs）31.4%和漆酶（Lac）40.4%。其比酶活分别为 30.2U/g（combi-CLEAs-LiPs）、9.5 U/g（combi-CLEAs-MnPs）和 28.4 U/g（combi-CLEAs-Lac）。此外，颗粒中 combi-CLEAs 的存在极大地提高了其降解染料的能力。与没有 combi-CLEAs 的固定化 Ph. chrysosporium 相比，共固定化颗粒提高了酸性紫 7（从 45.2%提高到 93.4%）和碱性品红（从 12.1%提高到 67.9%）的脱色率。此外，添加 combi-CLEAs 提高了白腐真菌颗粒对不利环境条件的适应性。
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