Chauhan Vivek, Kaushal Diksha, Dhiman Vivek Kumar, Kanwar Shamsher Singh, Singh Devendra, Dhiman Vinay Kumar, Pandey Himanshu
Department of Biotechnology, Himachal Pradesh University, Shimla, India.
B.N. College of Engineering and Technology, Lucknow, India.
Front Bioeng Biotechnol. 2022 Mar 2;10:794411. doi: 10.3389/fbioe.2022.794411. eCollection 2022.
Enzymes play vital roles in all organisms. The enzymatic process is progressively at its peak, mainly for producing biochemical products with a higher value. The immobilization of enzymes can sometimes tremendously improve the outcome of biocatalytic processes, making the product(s) relatively pure and economical. Carrier-free immobilized enzymes can increase the yield of the product and the stability of the enzyme in biocatalysis. Immobilized enzymes are easier to purify. Due to these varied advantages, researchers are tempted to explore carrier-free methods used for the immobilization of enzymes. In this review article, we have discussed various aspects of enzyme immobilization, approaches followed to design a process used for immobilization of an enzyme and the advantages and disadvantages of various common processes used for enzyme immobilization.
酶在所有生物体中都起着至关重要的作用。酶促过程逐渐达到顶峰，主要用于生产具有更高价值的生化产品。酶的固定化有时可以极大地改善生物催化过程的结果，使产品相对纯净且经济。无载体固定化酶可以提高生物催化中产品的产量和酶的稳定性。固定化酶更易于纯化。由于这些多样的优点，研究人员倾向于探索用于酶固定化的无载体方法。在这篇综述文章中，我们讨论了酶固定化的各个方面、设计酶固定化过程所遵循的方法以及各种常见酶固定化过程的优缺点。
