Key Laboratory of Applied Surface and Colloid Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University , Xi'an 710062, P. R. China.
ACS Appl Mater Interfaces. 2015 Oct 14;7(40):22487-96. doi: 10.1021/acsami.5b06604. Epub 2015 Oct 5.
A particular bispyrene fluorophore (1) with two pyrene moieties covalently linked via a hydrophilic spacer was synthesized. Fluorescence measurements reveal that the fluorescence emission of 1 could be well modulated by a cationic surfactant, dodecyltrimethylammonium bromide (DTAB). Protein sensing studies illustrate that the selected ensemble based on 1/DTAB assemblies exhibits ratiometric responses to nonmetalloproteins and turn-off responses to metalloproteins, which can be used to differentiate the two types of proteins. Moreover, negatively charged nonmetalloproteins can be discriminated from the positively charged ones according to the difference in ratiometric responses. Fluorescence sensing studies with control bispyrenes indicate that the polarity of the spacer connecting two pyrene moieties plays an important role in locating bispyrene fluorophore in DTAB assemblies, which further influences its sensing behaviors to noncovalent interacting proteins. This study sheds light on the influence of the probe structure on the sensing performance of a fluorescent ensemble based on probe and surfactant assemblies.
一种特殊的双芘荧光团（1），其两个芘部分通过亲水性间隔基共价连接，被合成出来。荧光测量显示，1 的荧光发射可以通过阳离子表面活性剂十二烷基三甲基溴化铵（DTAB）很好地调节。蛋白质传感研究表明，基于 1/DTAB 组装的选定体系对非金属蛋白酶表现出比率响应，对金属蛋白酶表现出关闭响应，可用于区分这两种类型的蛋白质。此外，根据比率响应的差异，可以区分带负电荷的非金属蛋白酶和带正电荷的蛋白酶。使用对照双芘的荧光传感研究表明，连接两个芘部分的间隔基的极性在将双芘荧光团定位在 DTAB 组装体中起着重要作用，这进一步影响了它对非共价相互作用蛋白质的传感行为。这项研究揭示了探针结构对基于探针和表面活性剂组装的荧光整体传感性能的影响。
