Mackinnon W B, Dyne M, Holmes K T, Mountford C E, Gupta R S
Department of Cancer Medicine, University of Sydney, NSW, Australia.
NMR Biomed. 1989 Nov;2(4):161-4. doi: 10.1002/nbm.1940020405.
1H magnetic resonance (MR) spectroscopy of intact viable malignant cells yields high resolution spectra from lipid. In previous studies we have provided evidence that these signals are generated by neutral lipid located in the plasma membrane in unique domains. We show that intracellular lipid droplets do not contribute to the MR signal. Two malignant Chinese hamster ovary cell lines, EOT and its parental line WT were studied. The EOT cells have a more highly resolved lipid spectrum than the WT, a result which correlates with slightly increased levels of triglyceride in highly purified plasma membranes. The intracellular lipid droplets of both lines were quantified using both fluorescence and electron microscopy but no significant differences were observed. Together these results provide evidence that narrow 1H MR signals from malignant cells arise from neutral lipid in the plasma membrane, rather than from intracellular lipid droplets.
完整存活的恶性细胞的氢磁共振（MR）波谱可产生来自脂质的高分辨率波谱。在先前的研究中，我们已提供证据表明，这些信号由位于独特结构域的质膜中的中性脂质产生。我们发现细胞内脂质小滴对MR信号没有贡献。研究了两种恶性中国仓鼠卵巢细胞系，EOT及其亲代细胞系WT。EOT细胞的脂质波谱比WT细胞的分辨率更高，这一结果与高度纯化的质膜中甘油三酯水平略有升高相关。使用荧光显微镜和电子显微镜对两个细胞系的细胞内脂质小滴进行了定量，但未观察到显著差异。这些结果共同提供了证据，表明恶性细胞的窄氢MR信号源自质膜中的中性脂质，而非细胞内脂质小滴。
