Callies R, Sri-Pathmanathan R M, Ferguson D Y, Brindle K M
Department of Biochemistry and Molecular Biology, University of Manchester, UK.
Magn Reson Med. 1993 Apr;29(4):546-50. doi: 10.1002/mrm.1910290418.
The appearance of high resolution neutral lipid signals in the 1H NMR spectra of myeloma cells grown in the presence of oleate was shown to correlate with the appearance of cytoplasmic lipid droplets observable by electron microscopy. The spin-spin relaxation times of these lipid signals were similar to those measured previously for lipid resonances in other cell types. These data suggest that cytoplasmic lipid droplets could make a significant contribution to the neutral lipid signals observed in the 1H NMR spectra of some cells.
在油酸存在下生长的骨髓瘤细胞的1H NMR谱中出现的高分辨率中性脂质信号，已被证明与通过电子显微镜观察到的细胞质脂滴的出现相关。这些脂质信号的自旋-自旋弛豫时间与先前在其他细胞类型中测量的脂质共振弛豫时间相似。这些数据表明，细胞质脂滴可能对某些细胞的1H NMR谱中观察到的中性脂质信号有显著贡献。
