Sun Cheng, Xu Jing, Song Jiaxi, Liu ChaoQun, Wang Jinyu, Weng Chenchun, Sun Haoyu, Wei Haiming, Xiao Weihua, Sun Rui, Tian Zhigang
Institute of Immunology and The CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, Anhui, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Oncotarget. 2015 Nov 3;6(34):35602-15. doi: 10.18632/oncotarget.5801.
The increasing evidences suggest that Immunoscore(IS), a combinatorial density analysis of CD8+ and CD3+ cells in the centre and invasive margin of tumour (CT and IM), has an advantage over the currently used tumour staging methods in a variety of tumours; however, IS in hepatocellular carcinoma remains unreported. In this study, IS was performed on serial sections from two HCC cohorts (total 449) and compared with current tumour staging systems. Kaplan-Meier curves illustrate a positive association between a higher IS (IS≥2) and longer survival of HCC patients. Although the IS was highly related to the outcome of patients, however, IS seems not to be the optimal prognostic factor when compared with the CD8CT. As noted, among CD8CT, CD8IM, CD3CT, CD3IM and IS, CD8CT, as an independent indicator, demonstrated the highest prognostic impact on both DFS and OS in our Cox multivariate regression analysis (P< 0.0001). In our study, the minimum cut-off value was 93 CD8CT cells per mm2, to be used to divide the patients into CD8CTHi group and CD8CTLo group in clinical settings. Our results suggest that CD8CT densities analysis notably improved the accuracy of survival prediction with convenience of clinical manipulation in HCC.
越来越多的证据表明，免疫评分（IS），即肿瘤中心和浸润边缘（CT和IM）CD8 +和CD3 +细胞的组合密度分析，在多种肿瘤中比目前使用的肿瘤分期方法更具优势；然而，肝细胞癌中的IS尚未见报道。在本研究中，对两个肝癌队列（共449例）的连续切片进行了IS检测，并与当前的肿瘤分期系统进行了比较。Kaplan-Meier曲线显示较高的IS（IS≥2）与肝癌患者较长的生存期呈正相关。虽然IS与患者的预后高度相关，但是，与CD8CT相比，IS似乎不是最佳的预后因素。如前所述，在CD8CT、CD8IM、CD3CT、CD3IM和IS中，CD8CT作为独立指标，在我们的Cox多因素回归分析中对DFS和OS均显示出最高的预后影响（P<0.0001）。在我们的研究中，最小临界值为每平方毫米93个CD8CT细胞，用于在临床中将患者分为CD8CTHi组和CD8CTLo组。我们的结果表明，CD8CT密度分析显著提高了肝癌生存预测的准确性，且临床操作方便。
