免疫蛋白质组学技术为弓形虫感染提供了一种非凡的诊断方法。

Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection.

作者信息

El-Ashram Saeed, Yin Qing, Barta John R, Khan Jamal, Liu Xianyong, Suo Xun

机构信息

National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.

出版信息

J Microbiol Methods. 2015 Dec;119:18-30. doi: 10.1016/j.mimet.2015.09.011. Epub 2015 Sep 28.

DOI:10.1016/j.mimet.2015.09.011

PMID:26415530

Abstract

Immunoproteomic technology offers an exceptional tool to fill the blanks that still exist in diagnosis of Toxoplasma gondii infection despite its annotated sequence. The pitfalls of serological assays and current immunoproteomic approaches are accentuated, and new approaches are presented to improve the signal and to eliminate the noise produced by blocking-specific background. This review also highlights examples where immunoproteomic studies have contributed to broaden our understanding of toxoplasmosis diagnosis. Further promising solutions, which immunoproteomic technology can grant for toxoplasmosis diagnosis are part of an intense discussion.

摘要

免疫蛋白质组学技术提供了一种卓越的工具，可填补尽管已有注释序列但在弓形虫感染诊断中仍然存在的空白。血清学检测和当前免疫蛋白质组学方法的缺陷被进一步凸显，同时还提出了新的方法来增强信号并消除由封闭特异性背景产生的噪音。本综述还重点介绍了免疫蛋白质组学研究有助于拓宽我们对弓形虫病诊断理解的实例。免疫蛋白质组学技术可为弓形虫病诊断提供的更具前景的解决方案是激烈讨论的一部分。