Institute of Marine Biology, Ocean College of Zhejiang University , Hangzhou, Zhejiang 310058, China.
Department of Pharmacology, School of Basic Medical Sciences, Zhejiang University , Hangzhou, Zhejiang 310058, China.
J Agric Food Chem. 2015 Nov 4;63(43):9504-12. doi: 10.1021/acs.jafc.5b03045. Epub 2015 Oct 21.
The present data showed that a natural compound isolated from the plant Physalis pubescens L. (Solanaceae), physapubescin B, exhibited antitumor activity against prostate cancer in vitro and in vivo. Treating prostate cancer cells with physapubescin B resulted in the accumulation of cells in the G2/M phase, which was associated with reduced Cdc25C levels and increased levels of CyclinB1, P21 as well as p-Cdk1 (Tyr15). Additionally, reactive oxygen species (ROS) generation was increased in physapubescin B-treated PC-3 cells. Furthermore, the physapubescin B-induced decrease of Cdc25C protein expression together with the G2/M phase cell cycle arrest were significantly abrogated by antioxidant NAC and GSH. Our data also demonstrated that physapubescin B exhibited strong in vivo antitumor efficacy in human prostate cancer PC3 xenograft. In conclusion, our results provide clear evidence that physapubescin B exhibits antitumor activity both in vitro and in vivo and deserves further development as an anticancer agent.
本研究数据表明，从茄科植物酸浆 Physalis pubescens L. 中分离得到的天然化合物 physapubescin B 具有体外和体内抗前列腺癌活性。用 physapubescin B 处理前列腺癌细胞导致细胞在 G2/M 期积累，这与 Cdc25C 水平降低以及 CyclinB1、P21 和 p-Cdk1（Tyr15）水平升高有关。此外，在 physapubescin B 处理的 PC-3 细胞中，活性氧 (ROS) 的产生增加。此外，抗氧化剂 NAC 和 GSH 显著阻断了 physapubescin B 诱导的 Cdc25C 蛋白表达减少和 G2/M 期细胞周期阻滞。我们的数据还表明，physapubescin B 在人前列腺癌 PC3 异种移植模型中表现出很强的体内抗肿瘤功效。总之，我们的研究结果提供了明确的证据，表明 physapubescin B 具有体外和体内抗肿瘤活性，值得进一步开发为抗癌药物。
