Patra Hirak K, Imani Roghayeh, Jangamreddy Jaganmohan R, Pazoki Meysam, Iglič Aleš, Turner Anthony P F, Tiwari Ashutosh
Biosensors and Bioelectronics Centre, IFM, Linköping University, 58183, Linköping, Sweden.
Integrative Regenerative Medicine Centre, Linköping University, 58185 Linköping, Linköping, Sweden.
Sci Rep. 2015 Sep 29;5:14571. doi: 10.1038/srep14571.
Throughout the world, there are increasing demands for alternate approaches to advanced cancer therapeutics. Numerous potentially chemotherapeutic compounds are developed every year for clinical trial and some of them are considered as potential drug candidates. Nanotechnology-based approaches have accelerated the discovery process, but the key challenge still remains to develop therapeutically viable and physiologically safe materials suitable for cancer therapy. Here, we report a high turnover, on/off-switchable functionally popping reactive oxygen species (ROS) generator using a smart mesoporous titanium dioxide popcorn (TiO2 Pops) nanoarchitecture. The resulting TiO2 Pops, unlike TiO2 nanoparticles (TiO2 NPs), are exceptionally biocompatible with normal cells. Under identical conditions, TiO2 Pops show very high photocatalytic activity compared to TiO2 NPs. Upon on/off-switchable photo activation, the TiO2 Pops can trigger the generation of high-turnover flash ROS and can deliver their potential anticancer effect by enhancing the intracellular ROS level until it crosses the threshold to open the 'death gate', thus reducing the survival of cancer cells by at least six times in comparison with TiO2 NPs without affecting the normal cells.
在全球范围内，对先进癌症治疗的替代方法的需求日益增加。每年都有大量潜在的化疗化合物被开发用于临床试验，其中一些被视为潜在的候选药物。基于纳米技术的方法加速了发现过程，但关键挑战仍然是开发适用于癌症治疗的具有治疗可行性和生理安全性的材料。在此，我们报告了一种使用智能介孔二氧化钛爆米花（TiO₂ Pops）纳米结构的高周转率、可开关功能的活性氧（ROS）发生器。与二氧化钛纳米颗粒（TiO₂ NPs）不同，所得的TiO₂ Pops与正常细胞具有出色的生物相容性。在相同条件下，与TiO₂ NPs相比，TiO₂ Pops表现出非常高的光催化活性。通过可开关的光激活，TiO₂ Pops可以触发高周转率的瞬时ROS生成，并通过提高细胞内ROS水平直至其超过阈值打开“死亡之门”来发挥其潜在的抗癌作用，从而与TiO₂ NPs相比，在不影响正常细胞的情况下，使癌细胞的存活率至少降低六倍。
