Xiao Bo, Liu Huazhen, Gu Zeyun, Liu Sining, Ji Cheng
School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.
Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, 224051, China.
Neurochem Res. 2015 Nov;40(11):2348-56. doi: 10.1007/s11064-015-1726-7. Epub 2015 Sep 28.
Cell transplantation of neural stem cells (NSCs) is a promising approach for neurological recovery both structurally and functionally. However, one big obstacle is to promote differentiation of NSCs into neurons and the followed maturation. In the present study, we aimed to investigate the protective effect of taurine on the differentiation of NSCs and subsequent maturation of their neuronal lineage, when exposed to oxygen-glucose deprivation (OGD). The results suggested that taurine (5-20 mM) promoted the viability and proliferation of NSCs, and it protected against 8 h of OGD induced impairments. Furthermore, 20 mM taurine promoted NSCs to differentiate into neurons after 7 days of culture, and it also protected against the suppressive impairments of 8 h of OGD. Consistently, taurine (20 mM) promoted the neurite sprouting and outgrowth of the NSC differentiated neurons after 14 days of differentiation, which were significantly inhibited by OGD (8 h). At D21, the mushroom spines and spine density were promoted or restored by 20 mM taurine. Taken together, the enhanced viability and proliferation of NSCs, more differentiated neurons and the promoted maturation of neurons by 20 mM taurine support its therapeutic application during stem cell therapy to enhance neurological recovery. Moreover, it protected against the impairments induced by OGD, which may highlight its role for a more direct therapeutic application especially in an ischemic stroke environment.
神经干细胞（NSCs）的细胞移植在结构和功能上都是促进神经功能恢复的一种有前景的方法。然而，一个大的障碍是促进神经干细胞向神经元分化及其随后的成熟。在本研究中，我们旨在研究牛磺酸在神经干细胞暴露于氧糖剥夺（OGD）时对其分化及随后神经元谱系成熟的保护作用。结果表明，牛磺酸（5 - 20 mM）可促进神经干细胞的活力和增殖，并保护其免受8小时氧糖剥夺诱导的损伤。此外，20 mM牛磺酸在培养7天后促进神经干细胞分化为神经元，并保护其免受8小时氧糖剥夺的抑制性损伤。同样，在分化14天后，牛磺酸（20 mM）促进神经干细胞分化的神经元的神经突萌发和生长，而这被8小时的氧糖剥夺显著抑制。在第21天，20 mM牛磺酸促进了蘑菇状棘突和棘突密度的增加或恢复。综上所述，20 mM牛磺酸增强了神经干细胞的活力和增殖，使更多神经元分化，并促进了神经元的成熟，这支持了其在干细胞治疗中用于促进神经功能恢复的治疗应用。此外，它保护细胞免受氧糖剥夺诱导的损伤，这可能突出了其在更直接治疗应用中的作用，特别是在缺血性中风环境中。
