Huang Hui-Ying, Tang Yi-Ju, King V An-Erl, Chou Jen-Wei, Tsen Jen-Horng
Department of Nutrition, China Medical University, Taichung, Taiwan, ROC.
Department of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, ROC.
Int Microbiol. 2015 Mar;18(1):61-9. doi: 10.2436/20.1501.01.235.
The protective effects of encapsulation on the survival of Lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. Viable counts and the remaining probiotic properties of calcium (Ca)-alginate encapsulated (A group), chitosan-Ca-alginate encapsulated (CA group), and unencapsulated, free L. reuteri (F group) were determined. Encapsulation improved the survival of L. reuteri subjected to simulated gastrointestinal conditions, with the greatest protective effect achieved in the CA group. The degree of cell membrane injury increased with increasing bile salt concentrations at constant pH, but the extent of injury was less in the encapsulated than in the free cells. Adherence rates were, in descending order: CA (0.524%)>A (0.360%)>F (0.275%). Lactobacillus reuteri cells retained their antagonistic activity toward Listeria monocytogenes even after incubation of the lactobacilli under simulated gastrointestinal conditions. Displacement of the pathogen by cells released from either of the encapsulation matrices was higher than that by free cells. The safety of L. reuteri was demonstrated in an in vitro invasion assay.
研究了包封对罗伊氏乳杆菌在模拟胃肠道条件下存活及保留该菌益生菌特性的保护作用。测定了海藻酸钙包封（A组）、壳聚糖 - 海藻酸钙包封（CA组）和未包封的游离罗伊氏乳杆菌（F组）的活菌数及剩余的益生菌特性。包封提高了罗伊氏乳杆菌在模拟胃肠道条件下的存活率，其中CA组的保护效果最佳。在恒定pH值下，细胞膜损伤程度随胆盐浓度的增加而增加，但包封细胞的损伤程度小于游离细胞。黏附率由高到低依次为：CA（0.524%）>A（0.360%）>F（0.275%）。即使在模拟胃肠道条件下培养后，罗伊氏乳杆菌细胞对单核细胞增生李斯特菌仍保持拮抗活性。来自任何一种包封基质释放的细胞对病原体的置换作用均高于游离细胞。在体外侵袭试验中证明了罗伊氏乳杆菌的安全性。
