Laboratorio de Bioconversiones, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto s/n, Col. La Laguna, Gustavo A. Madero, 07340, CDMX, Mexico.
Laboratorio de Biología Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, Col. La Escalera, Gustavo A. Madero, 07320, CDMX, Mexico.
Probiotics Antimicrob Proteins. 2024 Dec;16(6):2218-2228. doi: 10.1007/s12602-023-10166-3. Epub 2023 Oct 10.
Diabetes mellitus type 2 (DM2) is the most common chronic disease worldwide, characterized mainly by increased glucose concentration in the blood and affecting several organs' functionality. The daily consumption of probiotic bacteria can help control diabetes and reduce the damage caused. Cell immobilization techniques are a powerful tool that provides physical cell protection to such probiotic bacteria against gastrointestinal conditions. We suggest that cell immobilization could be a significant vector for delivering a high quantity of viable probiotics to the gut, helping attenuate hyperglycemia in diabetic rats. Seventy male Wistar rats were used in this work. Nicotinamide was administrated via intraperitoneal injection 15 minutes before inducing type 2 diabetes (DM2), followed by a second intraperitoneal injection of streptozotocin to induce DM2. Rats were divided into seven groups. For 45 days, a specific treatment was applied to each group. The group of rats, supplied with immobilized Lactobacillus casei, showed a serum glucose concentration of 137 mg/dL, which was close to the one observed in the groups of healthy rats (117 mg/dL) and rats treated with metformin (155 mg/dL). The diabetic rats without treatment presented a higher serum glucose concentration (461 mg/dL). In the rats treated with immobilized L. casei, there was no biochemical parameter alteration, and the cell morphology of the analyzed tissues was similar to those of the healthy group. The consumption of immobilized L. casei could allow a high quantity of viable probiotics to be delivered to the gut, reducing serum glucose concentration by up to 70% compared to diabetic rats and reducing organ damage caused by diabetes.
2 型糖尿病（DM2）是全球最常见的慢性疾病，主要特征为血液中葡萄糖浓度升高，并影响多个器官的功能。每天摄入益生菌可以帮助控制糖尿病并减少其造成的损害。细胞固定化技术是一种强大的工具，它可以为益生菌提供物理细胞保护，使其免受胃肠道环境的影响。我们提出，细胞固定化可能是将大量有活力的益生菌递送到肠道的重要载体，有助于减轻糖尿病大鼠的高血糖。本工作使用了 70 只雄性 Wistar 大鼠。在诱导 2 型糖尿病（DM2）之前，通过腹腔内注射烟酰胺 15 分钟，然后再进行第二次腹腔内注射链脲佐菌素以诱导 DM2。将大鼠分为 7 组。在 45 天的时间里，对每组应用特定的治疗方法。给予固定化干酪乳杆菌的大鼠血清葡萄糖浓度为 137mg/dL，接近健康大鼠（117mg/dL）和二甲双胍治疗大鼠（155mg/dL）的水平。未经治疗的糖尿病大鼠的血清葡萄糖浓度更高（461mg/dL）。在接受固定化 L. casei 治疗的大鼠中，没有生化参数改变，并且分析组织的细胞形态与健康组相似。固定化 L. casei 的摄入可以将大量有活力的益生菌递送到肠道，使血清葡萄糖浓度降低 70%，与糖尿病大鼠相比，减少了器官损伤。
