Di Bartolo Ilaria, Angeloni Giorgia, Monini Marina, Maione Ester, Marrone Raffaele, Ostanello Fabio, Ruggeri Franco Maria
Istituto Superiore di Sanità, Department of Veterinary Public Health and Food Safety, Rome, Italy.
Istituto Superiore di Sanità, Department of Veterinary Public Health and Food Safety, Rome, Italy.
Virus Res. 2016 Jan 4;211:89-95. doi: 10.1016/j.virusres.2015.09.014. Epub 2015 Sep 28.
In this study, we investigated hepatitis E virus (HEV) infection in piglets sampled in two farms in southern Italy. The virus was detected in 11 out of 15 animals tested. Based on sequence analysis, the 6 Italian strains examined belonged to two clusters containing both swine and human strains of either genotype 3 subtype e or f from Europe and Japan. The two Italian strain clusters shared nucleotide identity of 81.8% and 87.5% in the ORF2 (capsid protein) and ORF1 (RdRp) diagnostic fragments, respectively, confirming the heterogeneity of genotype 3 viruses circulating in pigs in Italy. The complete genome of one genotype 3 subtype e strain and the full ORF2 and ORF3 coding regions of one of the genotype 3f strains, obtained in this study, were compared to other HEV sequences available on line (NCBI database). The results of analysis showed that porcine strains clustered together with human and swine strains detected in Europe. Most changes in the coding region corresponded to synonymous mutations, and only the ORF3 showed a positive selection. Further, analyses are needed to understand the clinical significance of HEV genotypes and subtypes.
在本研究中，我们调查了意大利南部两个农场采集的仔猪的戊型肝炎病毒（HEV）感染情况。在检测的15只动物中，有11只检测到该病毒。基于序列分析，所检测的6株意大利毒株属于两个簇，包含来自欧洲和日本的3型e或f亚型的猪和人毒株。这两个意大利毒株簇在ORF2（衣壳蛋白）和ORF1（RdRp）诊断片段中的核苷酸同一性分别为81.8%和87.5%，证实了意大利猪中流行的3型病毒的异质性。将本研究中获得的一株3型e亚型毒株的全基因组以及一株3f型毒株的完整ORF2和ORF3编码区与在线（NCBI数据库）上的其他HEV序列进行了比较。分析结果表明，猪毒株与在欧洲检测到的人毒株和猪毒株聚集在一起。编码区的大多数变化对应于同义突变，只有ORF3显示出正选择。此外，需要进一步分析以了解HEV基因型和亚型的临床意义。
