Christie-Oleza Joseph A, Armengaud Jean
School of Life Sciences, University of Warwick, Coventry, UK.
CEA, DSV, IBiTec-S, SPI, Li2D, Laboratory "Innovative Technologies for Detection and Diagnostics", Bagnols-sur-Cèze, France.
Proteomics. 2015 Dec;15(23-24):3928-42. doi: 10.1002/pmic.201500222. Epub 2015 Nov 23.
Oceans are powered by metabolically-active microorganisms which are main drivers of global biogeochemical cycles on Earth. A decade ago, marine microbiology was boosted with next-generation sequencing capacities and the launch of large metagenomics surveys. High-performing proteomics is now comprehensive enough for reaching genome-wide and systems-wide scales. It is highly complementary to transcriptomics in order to analyze functional dynamics of marine microbes and microbial complex systems. Next-generation proteomics allows new perspectives for better understanding microbial lifestyles and uncovering the complexity of microbial communities. Here, we review the proteomics approaches and outcomes of recent work carried out on one of the most thoroughly studied marine generalist microorganisms, i.e. the Roseobacter clade, as pivotal examples. We also discuss how the study of the proteome of these organisms has helped in the understanding of the ecological strategy and lifestyle of this relevant marine clade, not only in laboratory cultures but also in its natural environment.
海洋由具有代谢活性的微生物驱动，这些微生物是地球上全球生物地球化学循环的主要驱动力。十年前，海洋微生物学因下一代测序能力以及大型宏基因组学调查的开展而得到推动。如今，高性能蛋白质组学已足够全面，能够达到全基因组和全系统规模。它与转录组学高度互补，用于分析海洋微生物和微生物复杂系统的功能动态。下一代蛋白质组学为更好地理解微生物生活方式和揭示微生物群落的复杂性提供了新视角。在此，我们以最深入研究的海洋泛养微生物之一——玫瑰杆菌属为例，综述蛋白质组学方法以及近期相关研究的成果。我们还将讨论对这些生物蛋白质组的研究如何有助于理解这一重要海洋类群的生态策略和生活方式，不仅是在实验室培养中，还包括其自然环境中。
