College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, PR China.
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, Maryland, USA.
Microb Genom. 2024 Apr;10(4). doi: 10.1099/mgen.0.001240.
The ubiquitous and abundant marine phages play critical roles in shaping the composition and function of bacterial communities, impacting biogeochemical cycling in marine ecosystems. is among the most abundant and ubiquitous phage families in the ocean. However, studies on the diversity and ecology of phages in marine environments are restricted to isolates that infect SAR11 bacteria and cyanobacteria. In this study, ten new roseophages that infect marine strains were isolated from coastal waters. These new roseophages have a genome size ranging from 38 917 to 42 634 bp and G+C content of 44.6-50 %. Comparative genomics showed that they are similar to known phages regarding gene content and architecture, thus representing the first roseophages. Phylogenomic analysis based on concatenated conserved genes showed that the ten roseophages form three distinct subgroups within the , and sequence analysis revealed that they belong to eight new genera. Finally, viromic read-mapping showed that these new phages are widely distributed in global oceans, mostly inhabiting polar and estuarine locations. This study has expanded the current understanding of the genomic diversity, evolution and ecology of phages and roseophages. We suggest that phages play important roles in the mortality and community structure of roseobacters, and have broad ecological applications.
海洋噬菌体无处不在且丰富多样，对塑造细菌群落的组成和功能起着关键作用，影响着海洋生态系统中的生物地球化学循环。 是海洋中最丰富和普遍的噬菌体家族之一。然而，海洋环境中 噬菌体的多样性和生态学研究仅限于感染 SAR11 细菌和蓝藻的分离株。在这项研究中，从沿海水域中分离到了 10 株新的感染海洋 菌株的玫瑰噬菌体。这些新的玫瑰噬菌体的基因组大小在 38917 到 42634bp 之间，GC 含量为 44.6-50%。比较基因组学表明，它们在基因内容和结构上与已知的 噬菌体相似，因此代表了第一个 玫瑰噬菌体。基于串联保守基因的系统发育基因组分析表明，这 10 株玫瑰噬菌体在 中形成三个不同的亚群，序列分析表明它们属于八个新属。最后，病毒组读数映射表明，这些新的 噬菌体广泛分布于全球海洋中，主要栖息在极地和河口地区。本研究扩展了对 噬菌体基因组多样性、进化和生态学的现有认识。我们认为 噬菌体在玫瑰杆菌的死亡率和群落结构中发挥着重要作用，并具有广泛的生态应用。
