Dees Merete Wiken, Lysøe Erik, Alsheikh Muath, Davik Jahn, Brurberg May Bente
NIBIO, The Norwegian Institute of Bioeconomy Research, 1430, Ås, Norway.
Graminor Breeding Ltd, 2322, Ridabu, Norway.
Mol Plant Pathol. 2016 Jun;17(5):703-13. doi: 10.1111/mpp.12323. Epub 2015 Dec 21.
Common scab, caused by species from the bacterial genus Streptomyces, is an important disease of potato (Solanum tuberosum) crops worldwide. Early tuberization is a critical period for pathogen infection; hence, studies of host gene expression responses during this developmental stage can be important to expand our understanding of the infection process and to identify putative resistance genes. In an infection experiment with the highly susceptible potato cultivar Saturna and the relatively resistant cultivar Beate, transcription profiles were obtained by RNA sequencing at two developmental stages: the early hook stage and the early tuber formation stage. Our results indicate that 'Beate' mounts an early and sustained response to infection by S. turgidiscabies, whereas the defence response by 'Saturna' ceases before the early tuber formation stage. Most pronounced were the putative candidate defence-associated genes uniquely expressed in 'Beate'. We observed an increase in alternative splicing on pathogen infection at the early hook stage for both cultivars. A significant down-regulation of genes involved in the highly energy-demanding process of ribosome biogenesis was observed for the infected 'Beate' plants at the early hook stage, which may indicate an allocation of resources that favours the expression of defence-related genes.
由链霉菌属细菌引起的普通疮痂病是全球马铃薯（Solanum tuberosum）作物的一种重要病害。块茎早期形成是病原体感染的关键时期；因此，研究宿主在这个发育阶段的基因表达反应对于扩展我们对感染过程的理解以及鉴定潜在的抗性基因可能很重要。在一项使用高度易感的马铃薯品种Saturna和相对抗性品种Beate进行的感染实验中，通过RNA测序在两个发育阶段获得了转录谱：早期钩状阶段和早期块茎形成阶段。我们的结果表明，“Beate”对肿胀链霉菌感染产生了早期且持续的反应，而“Saturna”的防御反应在早期块茎形成阶段之前就停止了。最显著的是在“Beate”中独特表达的假定候选防御相关基因。我们观察到两个品种在早期钩状阶段病原体感染时可变剪接增加。在早期钩状阶段，受感染的“Beate”植株中参与核糖体生物发生这一高能量需求过程的基因显著下调，这可能表明资源分配有利于防御相关基因的表达。
