de Figueiredo Sonia Maria, Binda Nancy Scardua, Almeida Bruno de Moura, Lemos Abreu Sheila Rago, Silva de Abreu José Alexandre, Pastore Gláucia Maria, Sato Hélia Harumi, Toreti Viviane Cristina, Tapia Eulália Vargas, Park Yong Kun, Vieira Filho Sidney Augusto, Caligiorne Rachel Basques
Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte (IEP/SCBH), Rua Domingos Vieira, 590, Belo Horizonte, 30150-240, Minas Gerais, Brazil.
Curr Drug Discov Technol. 2015;12(4):229-39. doi: 10.2174/1570163812666150929102420.
In order to verify the chemical qualities of polar extract of Green Propolis produced in the State of Minas Gerais, Brazil, was analyzed by means of RP-HPLC, the concentration of eleven flavonoids, Artepillin C and p-coumaric acid were analyzed by means of RP-HPLC. Samples were collected in the months of February, March, May, July, September, and October, during a period of six years (2008 to 2013) and the results compared with purchased standards. Artepillin C was the main constituent (80-90%) found in all samples. Lower concentrations of p-coumaric acid, chrysin and kaempferide were observed in March, and of ricin and galangin in September. Respectively, these months correspond to the end of Summer and Spring period in Brazil. The variation in concentration of the analyzed constituents always occurred in the same month, during the entire six years of evaluation period. All thirteen constituents of Green Propolis were detected throughout the study period, and the average concentration of each one was similar in relation to the respective monthly collection period of each year. Due to similarities among chemical constituents of Green Propolis with those present in B. dracuncufolia, this plant was identified as being the principal source of Green Propolis.
为验证巴西米纳斯吉拉斯州产绿蜂胶极性提取物的化学性质，采用反相高效液相色谱法（RP-HPLC）对11种黄酮类化合物、阿替匹林C和对香豆酸的浓度进行了分析。在2008年至2013年的六年时间里，于2月、3月、5月、7月、9月和10月采集样本，并将结果与购买的标准品进行比较。阿替匹林C是所有样本中的主要成分（80-90%）。3月观察到对香豆酸、白杨素和山奈酚的浓度较低，9月观察到蓖麻毒素和高良姜素的浓度较低。在巴西，这些月份分别对应夏末和春末时期。在整个六年的评估期内，所分析成分的浓度变化总是发生在同一个月。在整个研究期间检测到了绿蜂胶的所有13种成分，并且每种成分的平均浓度与每年各自的月度采集期相似。由于绿蜂胶的化学成分与龙脑香科植物中的化学成分相似，这种植物被确定为绿蜂胶的主要来源。
