Chekkoury Andrei, Gateau Jérôme, Driessen Wouter, Symvoulidis Panagiotis, Bézière Nicolas, Feuchtinger Annette, Walch Axel, Ntziachristos Vasilis
Chair for Biologial Imaging, Technische Universität München (TUM), Ismaningerstr. 22, 81675, Munich, Germany ; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany.
Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany ; iThera Medical, GmbH, Zielstattstraße 13, 81379, Munich, Germany.
Biomed Opt Express. 2015 Jul 31;6(9):3134-48. doi: 10.1364/BOE.6.003134. eCollection 2015 Sep 1.
Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz-40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2B-MSOM) superior to previous optical or optoacoustic mesoscopy implementations.
光学断层扫描术将生物可视化的能力扩展到了显微镜所能达到的有限穿透深度之外。然而，对于不透明生物或尺寸超过几百微米的组织进行成像，需要对标本进行侵入性组织切片或化学处理以清除光子散射，而这种侵入性过程在深度方面仍存在限制。我们开发了此前未报道的宽带光声断层扫描术作为一种断层成像方式，能够在无需对组织进行化学处理的情况下，对几毫米厚的组织进行光学对比度成像。我们表明，在500kHz至40MHz的宽频率范围内进行三维投影并结合多波长照明的独特组合，是使宽带多光谱光声断层扫描术（2B-MSOM）优于以往光学或光声断层扫描术的必要条件。
