Lee Jung-Su, Bae Young-Min, Lee Sook-Young, Lee Sun-Young
Dept. of Food Science and Technology, Chung-Ang Univ, 72-1 Nae-ri, Daedeok-myeon, Anseong-si, Gyeonggi-do, 456-756, South Korea.
J Food Sci. 2015 Oct;80(10):M2279-86. doi: 10.1111/1750-3841.13017. Epub 2015 Sep 28.
This study investigated the effect of material types (polystyrene, polypropylene, glass, and stainless steel) and glucose addition on Staphylococcus aureus biofilm formation, and the relationship between biofilm formation measured by crystal violet (CV) staining and the number of biofilm cells determined by cell counts was studied. We also evaluated the efficacy of chlorine sanitizer on inhibiting various different types of S. aureus biofilms on the surface of stainless steel. Levels of biofilm formation of S. aureus were higher on hydrophilic surfaces (glass and stainless steel) than on hydrophobic surfaces (polypropylene and polystyrene). With the exception of biofilm formed on glass, the addition of glucose in broth significantly increased the biofilm formation of S. aureus on all surfaces and for all tested strains (P ≤ 0.05). The number of biofilm cells was not correlated with the biomass of the biofilms determined using the CV staining method. The efficacy of chlorine sanitizer against biofilm of S. aureus was not significantly different depending on types of biofilm (P > 0.05). Therefore, further studies are needed in order to determine an accurate method quantifying levels of bacterial biofilm and to evaluate the resistance of bacterial biofilm on the material surface.
Biofilm formation of Staphylococcus aureus on the surface was different depending on the surface characteristics and S. aureus strains. There was low correlation between crystal violet staining method and viable counts technique for measuring levels of biofilm formation of S. aureus on the surfaces. These results could provide helpful information for finding and understanding the quantification method and resistance of bacterial biofilm on the surface.
本研究调查了材料类型（聚苯乙烯、聚丙烯、玻璃和不锈钢）以及添加葡萄糖对金黄色葡萄球菌生物膜形成的影响，并研究了通过结晶紫（CV）染色测定的生物膜形成与通过细胞计数确定的生物膜细胞数量之间的关系。我们还评估了氯消毒剂对抑制不锈钢表面各种不同类型金黄色葡萄球菌生物膜的效果。金黄色葡萄球菌在亲水性表面（玻璃和不锈钢）上的生物膜形成水平高于疏水性表面（聚丙烯和聚苯乙烯）。除了在玻璃上形成的生物膜外，肉汤中添加葡萄糖显著增加了所有表面上所有测试菌株的金黄色葡萄球菌生物膜形成（P≤0.05）。生物膜细胞数量与使用CV染色法测定的生物膜生物量不相关。氯消毒剂对金黄色葡萄球菌生物膜的效果因生物膜类型而异，差异不显著（P>0.05）。因此，需要进一步研究以确定准确的细菌生物膜定量方法，并评估材料表面细菌生物膜的抗性。
金黄色葡萄球菌在表面的生物膜形成因表面特性和金黄色葡萄球菌菌株而异。在测量金黄色葡萄球菌在表面的生物膜形成水平时，结晶紫染色法与活菌计数技术之间的相关性较低。这些结果可为寻找和理解表面细菌生物膜的定量方法及抗性提供有用信息。
