利用兔回肠袢模型对福氏志贺菌2a菌株301进行比较蛋白质组学研究，揭示了细菌在宿主生态位中适应的关键蛋白。
Comparative proteomics of Shigella flexneri 2a strain 301 using a rabbit ileal loop model reveals key proteins for bacterial adaptation in host niches.
作者信息
Liu Xingming, Lu Lilan, Liu Xiankai, Pan Chao, Feng Erling, Wang Dongshu, Zhu Li, Wang Hengliang
机构信息
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
出版信息
Int J Infect Dis. 2015 Nov;40:28-33. doi: 10.1016/j.ijid.2015.09.014. Epub 2015 Sep 28.
OBJECTIVES
Many studies focusing on changes in the host following Shigella spp invasion have been reported in recent years. However, the key factors required for the adaptation of these pathogens to host niches have usually been neglected.
METHODS
In this study, a comparative proteomic analysis was performed to examine changes in the protein expression profile of Shigella flexneri within the host using a rabbit ileal loop model to reveal proteins that are associated with pathogenic adaptation.
RESULTS
The protein expression profiles of bacteria isolated from the ileum and colon were very similar, although they differed slightly from that of bacteria isolated from the cecum. When compared with the sample in vitro, the expressions of seven proteins were found to be upshifted in vivo (OmpA, YgiW, MglB, YfiD, MetK, TktA, and AhpF), while two proteins were down-regulated (ElaB and GlnH).
CONCLUSIONS
The abundance of nine proteins changed in vivo, suggesting that these proteins may contribute to adaptation to the intestinal lumen.
目的
近年来，已有许多关于志贺氏菌属入侵后宿主变化的研究报道。然而，这些病原体适应宿主生态位所需的关键因素通常被忽视。
方法
在本研究中，利用兔回肠袢模型进行比较蛋白质组学分析，以检测弗氏志贺氏菌在宿主体内的蛋白质表达谱变化，从而揭示与致病适应性相关的蛋白质。
结果
从回肠和结肠分离的细菌的蛋白质表达谱非常相似，尽管与从盲肠分离的细菌略有不同。与体外样本相比，发现有7种蛋白质在体内表达上调（OmpA、YgiW、MglB、YfiD、MetK、TktA和AhpF），而有2种蛋白质表达下调（ElaB和GlnH）。
结论
9种蛋白质的丰度在体内发生了变化，表明这些蛋白质可能有助于适应肠腔环境。
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