Silva Wanderson M, Carvalho Rodrigo D De Oliveira, Dorella Fernanda A, Folador Edson L, Souza Gustavo H M F, Pimenta Adriano M C, Figueiredo Henrique C P, Le Loir Yves, Silva Artur, Azevedo Vasco
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas GeraisBelo Horizonte, Brazil.
Institut National de la Recherche Agronomique (INRA), UMR1253 Science & Technologie du Lait & de l'Oeuf (STLO)Rennes, France.
Front Cell Infect Microbiol. 2017 Jul 25;7:325. doi: 10.3389/fcimb.2017.00325. eCollection 2017.
biovar is the etiologic agent of ulcerative lymphangitis. To investigate proteins that could be related to the virulence of this pathogen, we combined an experimental passage process using a murine model and high-throughput proteomics with a mass spectrometry, data-independent acquisition (LC-MS) approach to identify and quantify the proteins released into the supernatants of strain 258. To our knowledge, this approach allowed characterization of the exoproteome of a strain for the first time. Interestingly, the recovery of this strain from infected mouse spleens induced a change in its virulence potential, and it became more virulent in a second infection challenge. Proteomic screening performed from culture supernatant of the control and recovered conditions revealed 104 proteins that were differentially expressed between the two conditions. In this context, proteomic analysis of the recovered condition detected the induction of proteins involved in bacterial pathogenesis, mainly related to iron uptake. In addition, KEGG enrichment analysis showed that ABC transporters, bacterial secretion systems and protein export pathways were significantly altered in the recovered condition. These findings show that secretion and secreted proteins are key elements in the virulence and adaptation of . Collectively, bacterial pathogenesis-related proteins were identified that contribute to the processes of adherence, intracellular growth and evasion of the immune system. Moreover, this study enhances our understanding of the factors that may influence the pathogenesis of .
生物变种是溃疡性淋巴管炎的病原体。为了研究可能与这种病原体毒力相关的蛋白质，我们将使用小鼠模型的实验传代过程和高通量蛋白质组学与质谱数据非依赖采集（LC-MS）方法相结合，以鉴定和定量释放到258菌株上清液中的蛋白质。据我们所知，这种方法首次实现了对一个菌株外蛋白质组的表征。有趣的是，从感染小鼠脾脏中回收该菌株后，其毒力潜力发生了变化，并且在第二次感染挑战中变得更具毒力。对对照条件和回收条件下的培养上清液进行蛋白质组学筛选，发现两种条件下有104种蛋白质差异表达。在此背景下，对回收条件进行蛋白质组学分析检测到参与细菌致病过程的蛋白质的诱导，主要与铁摄取有关。此外，KEGG富集分析表明，在回收条件下ABC转运蛋白、细菌分泌系统和蛋白质输出途径发生了显著改变。这些发现表明，分泌蛋白和分泌过程是该病原体毒力和适应性的关键因素。总的来说，鉴定出了与细菌致病相关的蛋白质，它们有助于病原体的黏附、细胞内生长和免疫系统逃避过程。此外，这项研究增进了我们对可能影响该病原体致病机制的因素的理解。
