Rubinstien E, Ballow M
Department of Medicine, Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105.
J Clin Lab Immunol. 1989 Sep;30(1):35-9.
Utilizing a recently reported characteristic of monocytes to aggregate in cold, a new procedure for obtaining monocyte-rich and monocyte-depleted mononuclear fractions from human blood is described. After aggregation in cold and adherence to plastic, a fraction containing 88% monocyte (LeuM3 CD14+) is obtained. After treating the supernatant, which separates from the aggregates, with a monocyte lysosomotropic agent (L-Leucine Methyl Ester), a fraction containing 0% monocyte (LeuM3 CD14+) and 88% lymphocytes (Leu4 CD3+ and Leu 12 CD19+) is obtained. In round-bottom culture wells, this monocyte-depleted fraction produced immunoglobulins in response to pokeweed mitogen (5,826 +/- 2,356 IgM micrograms/ml), to a degree not significantly different that that produced in the presence of monocytes (7,426 +/- 3,347 IgM micrograms/ml). This suggests the presence of cells in the lymphocyte fraction, that are not monocytes but are capable of antigen presenting.
利用最近报道的单核细胞在低温下聚集的特性，描述了一种从人血中获得富含单核细胞和单核细胞耗竭的单核细胞组分的新方法。在低温下聚集并贴壁于塑料后，可获得含有88%单核细胞（LeuM3 CD14+）的组分。在用单核细胞溶酶体亲和剂（L-亮氨酸甲酯）处理与聚集体分离的上清液后，可获得含有0%单核细胞（LeuM3 CD14+）和88%淋巴细胞（Leu4 CD3+和Leu 12 CD19+）的组分。在圆底培养孔中，这种单核细胞耗竭的组分对商陆有丝分裂原产生免疫球蛋白反应（5826±2356μg/ml IgM），其程度与在有单核细胞存在时产生的程度无显著差异（7426±3347μg/ml IgM）。这表明淋巴细胞组分中存在非单核细胞但能够呈递抗原的细胞。
