Astiazarán A M, Chico Y, Ruiz M B, Alonso M T, Ochoa B, Lacort M
Departamento de Fisiología, Facultad de Medicina y Odontología, Universidad del País Vasco, Bilbao, Spain.
Rev Esp Fisiol. 1989;45 Suppl:19-24.
Short-term effects of estradiol on gluconeogenesis, redox state and on the activities of the enzymes involved in NADPH production have been examined. Hepatocytes incubated with estradiol (10(-4)M) showed a decreased gluconeogenesis and an increased lactate/pyruvate ratio. The malic enzyme was found to be stimulated by 45%, whereas glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activities were not affected by the presence of the hormone. Estradiol produced selective inhibitions of glucose synthesis from various substrates and diminished malate dehydrogenase activity by 22%. The possibility that the estradiol-induced alterations here reported are related to the hormone catabolism itself in the liver is suggested. Other results in this work call attention to the importance of the vehicle used for the steroid dispersion. Propylene glycol markedly alters the metabolic state of liver cells and also antagonizes the modifications produced by estradiol.
已研究了雌二醇对糖异生、氧化还原状态以及参与烟酰胺腺嘌呤二核苷酸磷酸（NADPH）生成的酶活性的短期影响。用雌二醇（10⁻⁴M）孵育的肝细胞显示糖异生减少，乳酸/丙酮酸比值增加。发现苹果酸酶受到45%的刺激，而葡萄糖-6-磷酸脱氢酶和异柠檬酸脱氢酶的活性不受该激素存在的影响。雌二醇对从各种底物合成葡萄糖产生选择性抑制，并使苹果酸脱氢酶活性降低22%。本文报道的雌二醇诱导的改变可能与肝脏中激素分解代谢本身有关。这项工作的其他结果提醒人们注意用于甾体分散的载体的重要性。丙二醇会显著改变肝细胞的代谢状态，并且还会拮抗雌二醇产生的修饰作用。
