增殖性糖尿病视网膜病变中玻璃体内WNT3A增加与血管内皮生长因子的相关性

CORRELATION OF INCREASED INTRAVITREOUS WNT3A WITH VASCULAR ENDOTHELIAL GROWTH FACTOR IN PROLIFERATIVE DIABETIC RETINOPATHY.

作者信息

Gao Xinxiao, Ma Kai, Lu Ning, Hong Tingting, Xu Yongsheng, Peng Xiaoyan

机构信息

*Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Laboratory, Beijing, China; †Department of Ophthalmology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China; ‡Department of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China; and §Clinical Lab of Tissue and Cell Research Center, Department of Biotech Treatment, Logistics College of Chinese People's Armed Police Force, Tianjin, China.

出版信息

Retina. 2016 Apr;36(4):812-8. doi: 10.1097/IAE.0000000000000784.

DOI:10.1097/IAE.0000000000000784

PMID:26418446

Abstract

PURPOSE

To investigate Wnt3a and vascular endothelial growth factor (VEGF) levels in the vitreous fluid of patients with proliferative diabetic retinopathy (PDR) and to examine their correlation with PDR activity.

METHODS

Vitreous samples from 45 eyes with PDR and 28 eyes with nondiabetic macular disease were collected. Active PDR was present in 24 patients and inactive PDR in 21 patients, according to retinal neovascularization. The Wnt3a and VEGF level of vitreous fluid samples were measured by enzyme-linked immunosorbent assay.

RESULTS

Comparison revealed that mean intravitreal levels of Wnt3a increased significantly in PDR eyes compared with control eyes (13.55 ng/mL vs. 1.57 ng/mL, P < 0.001). The mean VEGF concentrations in the vitreous fluid of patients with PDR were also higher than those in nondiabetic controls, with the values being 723.21 pg/mL and 20.81 pg/mL, respectively (P < 0.001). In addition, vitreous concentrations of Wnt3a and VEGF were significantly higher in active PDR than in eyes with inactive PDR (P = 0.016 and P = 0.008, respectively). Furthermore, a significant positive correlation was detected between Wnt3a and VEGF levels in the vitreous.

CONCLUSION

Intravitreous levels of Wnt3a and VEGF in patients with PDR are increased and correlated mutually. Wnt3a may be an important player in the development of diabetic retinopathy and its activity in vitreous fluid can be biomarker of PDR.

摘要

目的

研究增殖性糖尿病视网膜病变（PDR）患者玻璃体液中Wnt3a和血管内皮生长因子（VEGF）水平，并探讨其与PDR活动度的相关性。

方法

收集45例PDR患者的45只眼和28例非糖尿病性黄斑疾病患者的28只眼的玻璃体样本。根据视网膜新生血管情况，24例患者为活动性PDR，21例患者为非活动性PDR。采用酶联免疫吸附测定法测量玻璃体样本中Wnt3a和VEGF水平。

结果

比较发现，与对照眼相比，PDR患眼玻璃体内Wnt3a平均水平显著升高（13.55 ng/mL对1.57 ng/mL，P<0.001）。PDR患者玻璃体液中的平均VEGF浓度也高于非糖尿病对照组，分别为723.21 pg/mL和20.81 pg/mL（P<0.001）。此外，活动性PDR患眼玻璃体内Wnt3a和VEGF浓度显著高于非活动性PDR患眼（分别为P=0.016和P=0.008）。此外，玻璃体内Wnt3a和VEGF水平之间存在显著正相关。