Künnecke B, Cerdan S
Biocenter of the University of Basel, Switzerland.
NMR Biomed. 1989 Dec;2(5-6):274-7. doi: 10.1002/nbm.1940020516.
The potential use of 13C multilabeled substrates has been studied in biological applications using in vivo and in vitro proton and 13C NMR spectroscopy. In 13C NMR spectroscopy, multilabeled compounds allow the simultaneous observation of several nuclei or increase distinctly the signal to noise ratio due to a higher degree of enrichment. Contiguous labeling of substrates leads to homonuclear 13C-13C spin couplings and provides a simple means to distinguish between endogenous stores of metabolites and metabolites derived from added substrates.
13C多标记底物在生物应用中的潜在用途已通过体内和体外质子及13C核磁共振光谱进行了研究。在13C核磁共振光谱中，多标记化合物能够同时观察多个原子核，或者由于更高的富集度而显著提高信噪比。底物的连续标记会导致同核13C-13C自旋耦合，并提供了一种简单的方法来区分代谢物的内源性储存和添加底物衍生的代谢物。
